Validation Data Gallery
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Tested Applications
| Positive WB detected in | human brain tissue, human placenta tissue, MCF-7 cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human oesophagus tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IHC | See 3 publications below |
Product Information
12634-1-AP targets DIRAS1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DIRAS1 fusion protein Ag3329 相同性解析による交差性が予測される生物種 |
| Full Name | DIRAS family, GTP-binding RAS-like 1 |
| Calculated molecular weight | 198 aa, 22 kDa |
| Observed molecular weight | 22-24 kDa |
| GenBank accession number | BC030660 |
| Gene Symbol | DIRAS1 |
| Gene ID (NCBI) | 148252 |
| RRID | AB_2292771 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95057 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DIRAS1 belongs to the small guanosine triphosphatase (GTPase) Ras superfamily. DIRAS1 is frequently downregulated in human cancers due to DNA copy number loss, loss of heterozygosity (LOH), and hypermethylation of the promoter. The restoration of DIRAS1 has been reported to suppress tumor growth and metastasis in human neural tumors, esophageal cancer, colorectal cancer, and ovarian cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DIRAS1 antibody 12634-1-AP | Download protocol |
| IHC protocol for DIRAS1 antibody 12634-1-AP | Download protocol |
| IP protocol for DIRAS1 antibody 12634-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancers (Basel) Frequent Epigenetic Inactivation of DIRAS-1 and DIRAS-2 Contributes to Chemo-Resistance in Gliomas. | ||
Mol Ther Nucleic Acids RNAa and Vector-Mediated Overexpression of DIRAS1 Suppresses Tumor Growth and Migration in Renal Cell Carcinoma.
| ||
J Biol Chem The Tumor Suppressive Small GTPase DiRas1 Binds the Non-canonical Guanine Nucleotide Exchange Factor SmgGDS and Antagonizes SmgGDS Interactions with Oncogenic Small GTPases. | ||
Int J Environ Res Public Health Effects of In Utero Exposure to Di-n-Butyl Phthalate on Testicular Development in Rat. | ||
Cancer Biol Ther M6A modification regulates tumor suppressor DIRAS1 expression in cervical cancer cells |