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KD/KO Validated

DIRAS2 Polyclonal antibody

Name: DIRAS2 Polyclonal antibody
Brand: Proteintech
SKU: 15557-1-AP

DIRAS2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15557-1-AP

Synonyms

GTP-binding protein Di-Ras2, GTP binding protein Di Ras2

Validation Data Gallery

Filter:

AllWBIPIF/ICC

WB analysis of human brain using 15557-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 15557-1-AP (DIRAS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15557-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15557-1-AP (DIRAS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 15557-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15557-1-AP (DIRAS2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 15557-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 15557-1-AP (DIRAS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 15557-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15557-1-AP (DIRAS2 antibody) at dilution of 1:1500 incubated at 4 degree celsius over night.
IP experiment of mouse brain using 15557-1-AP
IP result of anti-DIRAS2 (IP:15557-1-AP, 4ug; Detection:15557-1-AP 1:1000) with mouse brain tissue lysate 4400ug.
IF Staining of HeLa using 15557-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15557-1-AP (DIRAS2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 15557-1-AP
Immunofluorescent analysis of Hela cells, using DIRAS2 antibody 15557-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Cat No. 15557-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	human brain tissue, rat brain tissue, HeLa cells, mouse brain tissue
Positive IP detected in	mouse brain tissue
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

15557-1-AP targets DIRAS2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DIRAS2 fusion protein Ag7926 相同性解析による交差性が予測される生物種
Full Name	DIRAS family, GTP-binding RAS-like 2
Calculated molecular weight	22 kDa
Observed molecular weight	22 kDa
GenBank accession number	BC008065
Gene symbol	DIRAS2
Gene ID (NCBI)	54769
RRID	AB_2261563
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for DIRAS2 antibody 15557-1-AP	Download protocol
IF protocol for DIRAS2 antibody 15557-1-AP	Download protocol
IP protocol for DIRAS2 antibody 15557-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IP	Oncogene Di-Ras2 promotes renal cell carcinoma formation by activating the mitogen-activated protein kinase pathway in the absence of von Hippel-Lindau protein. Authors - Hanyu Rao KD Validated View Article
human	WB	Cancers (Basel) Frequent Epigenetic Inactivation of DIRAS-1 and DIRAS-2 Contributes to Chemo-Resistance in Gliomas. Authors - Tanja Rothhammer-Hampl View Article
human,mouse	WB,IF	J Neural Transm (Vienna) Expression of the ADHD candidate gene Diras2 in the brain. Authors - Lena Grünewald View Article
human	WB	Dev Cell The UBE2F-CRL5ASB11-DIRAS2 axis is an oncogene and tumor suppressor cascade in pancreatic cancer cells Authors - Yu Chang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DIRAS2 Polyclonal antibody

DIRAS2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of human brain using 15557-1-AP

WB analysis of HeLa using 15557-1-AP

WB analysis of mouse brain using 15557-1-AP

WB analysis of rat brain using 15557-1-AP

WB analysis using 15557-1-AP

IP experiment of mouse brain using 15557-1-AP

IF Staining of HeLa using 15557-1-AP

IF Staining of HeLa using 15557-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Di-Ras2 promotes renal cell carcinoma formation by activating the mitogen-activated protein kinase pathway in the absence of von Hippel-Lindau protein.

Frequent Epigenetic Inactivation of DIRAS-1 and DIRAS-2 Contributes to Chemo-Resistance in Gliomas.

Expression of the ADHD candidate gene Diras2 in the brain.

The UBE2F-CRL5ASB11-DIRAS2 axis is an oncogene and tumor suppressor cascade in pancreatic cancer cells