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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DIS3L Polyclonal antibody

DIS3L Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 25746-1-AP

Synonyms

DIS3 like exonuclease 1, DIS3L, DIS3L1, KIAA1955

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using DIS3L Polyclonal antibody (25746-1-AP)

    WB analysis of K-562 using 25746-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 25746-1-AP (DIS3L Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using DIS3L Polyclonal antibody (25746-1-AP)

    IP experiment of K-562 using 25746-1-AP

    IP result of anti-DIS3L (IP:25746-1-AP, 4ug; Detection:25746-1-AP 1:300) with K-562 cells lysate 3200ug.

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Tested Applications

Positive WB detected inK-562 cells
Positive IP detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 25746-1-AP is DIS3L Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DIS3L fusion protein Ag22689 相同性解析による交差性が予測される生物種
Full Name DIS3 mitotic control homolog (S. cerevisiae)-like
Observed molecular weight 121 kDa
GenBank accession numberBC022089
Gene symbol DIS3L
Gene ID (NCBI) 115752
RRIDAB_2880222
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DIS3L antibody 25746-1-APDownload protocol
IP protocol for DIS3L antibody 25746-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Life Sci Alliance

Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication

Authors - Tobias Moll
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Tsimafei (Verified Customer) (08-07-2024)

Antibody specifically recognize Dis3L

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mESCs
DIS3L Antibody Western Blot validation (1:1000 dilution) in mESCs (Cat no:25746-1-AP)