Validation Data Gallery
Tested Applications
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
15500-1-AP targets DISC1-long-specific in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | disrupted in schizophrenia 1 |
| Calculated molecular weight | 95 kDa |
| GenBank accession number | NM_018662 |
| Gene Symbol | DISC1 |
| Gene ID (NCBI) | 27185 |
| RRID | AB_2230451 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NRI5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DISC1, also named as KIAA0457, is identified as the sole gene whose ORF is truncated and cosegregates with major mental illnesses in a Scottish family. DISC1 may be implicated in psychiatric conditions in other populations than the unique Scottish family. It is involved in the regulation of multiple aspects of embryonic and adult neurogenesis. DISC1 is required for neural progenitor proliferation in the ventrical/subventrical zone during embryonic brain development and in the adult dentate gyrus of the hippocampus. It plays a role, together with PCNT, in the microtubule network formation. In WB, DISC1 has some MW such as 34kd(PMID:18620078 );75-85kd and 95-100kd(PMID:15657124). DISC1 isoforms have distinct developmental patterns of expression. This antibody is against C-terminal of DISC1, binding full length protein specifically.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for DISC1-long-specific antibody 15500-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Cell Neurosci Lack of Helios During Neural Development Induces Adult Schizophrenia-Like Behaviors Associated With Aberrant Levels of the TRIF-Recruiter Protein WDFY1. | ||
Neurobiol Dis Hippocampal Pyk2 regulates specific social skills: Implications for schizophrenia |