Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, mouse brain tissue, K-562 cells, HeLa cells, mouse heart tissue, rat brain tissue, rat heart tissue |
| Positive IHC detected in | human gliomas tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 37 publications below |
| IHC | See 11 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
Product Information
21112-1-AP targets DKK1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, rabbit |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DKK1 fusion protein Ag15110 相同性解析による交差性が予測される生物種 |
| Full Name | dickkopf homolog 1 (Xenopus laevis) |
| Calculated molecular weight | 266 aa, 29 kDa |
| Observed molecular weight | 30-35 kDa |
| GenBank accession number | BC001539 |
| Gene Symbol | DKK1 |
| Gene ID (NCBI) | 22943 |
| RRID | AB_10733097 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O94907 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DKK1, also named a SK and Dickkopf-1, belongs to the dickkopf family. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. SDS-PAGE and Western blot analysis demonstrated that DKK1 is expressed as a 35-kDa doublet protein, which is larger than the deduced molecular mass of 26 kDa. Sometime DKK1 is expressed as a 42- to 50-kDa secreted protein, with little change observed after glycanase treatment.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DKK1 antibody 21112-1-AP | Download protocol |
| IHC protocol for DKK1 antibody 21112-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Cancer stem cell regulated phenotypic plasticity protects metastasized cancer cells from ferroptosis.
| ||
Cell Death Dis Enhancing immunotherapy efficacy in colorectal cancer: targeting the FGR-AKT-SP1-DKK1 axis with DCC-2036 (Rebastinib) | ||
Bioengineered Exosomes from bone marrow mesenchymal stem cells promoted osteogenic differentiation by delivering miR-196a that targeted Dickkopf-1 to activate Wnt/β-catenin pathway | ||
Oncotarget MicroRNA-372 inhibits endometrial carcinoma development by targeting the expression of the Ras homolog gene family member C (RhoC). | ||
Osteoporos Int Rehmanniae Radix Preparata suppresses bone loss and increases bone strength through interfering with canonical Wnt/β-catenin signaling pathway in OVX rats. |