Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 13426-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13426-1-AP (DLAT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 13426-1-AP
WB result of DLAT antibody (13426-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DLAT transfected HeLa cells.
WB analysis using 13426-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13426-1-AP (DLAT antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.
WB analysis of HAP1 using 13426-1-AP
WB result of DLAT antibody (13426-1-AP; 1:60000; room temperature for 1.5 hours) with wild-type and DLAT knockout HAP1 cells.
IP experiment of HepG2 using 13426-1-AP
IP result of anti-DLAT (IP:13426-1-AP, 3ug; Detection:13426-1-AP 1:1000) with HepG2 cells lysate 2000ug.
IHC staining of human liver cancer using 13426-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13426-1-AP (DLAT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 13426-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13426-1-AP (DLAT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 13426-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13426-1-AP (DLAT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 13426-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13426-1-AP (DLAT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 13426-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13426-1-AP (DLAT antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 13426-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DLAT antibody (13426-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HepG2 using 13426-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DLAT antibody (13426-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

Featured Product
KD/KO Validated

DLAT Polyclonal antibody

DLAT Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13426-1-AP

Publications(70)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

PDC-E2, ODP2, EC:2.3.1.12, DLTA, Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (70)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, A431 cells, HepG2 cells, Jurkat cells, LNCaP cells, MCF-7 cells, HEK-293 cells
Positive IP detected in	HepG2 cells
Positive IHC detected in	human liver cancer tissue, human colon cancer tissue, mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 49 publications below
IHC	See 17 publications below
IF	See 19 publications below
IP	See 1 publications below
CoIP	See 2 publications below

Product Information

13426-1-AP targets DLAT in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, rabbit, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DLAT fusion protein Ag4226 相同性解析による交差性が予測される生物種
Full Name	dihydrolipoamide S-acetyltransferase
Calculated molecular weight	647 aa, 70 kDa
Observed molecular weight	69-70 kDa
GenBank accession number	BC039084
Gene Symbol	DLAT
Gene ID (NCBI)	1737
RRID	AB_2091774
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P10515
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for DLAT antibody 13426-1-AP	Download protocol
IHC protocol for DLAT antibody 13426-1-AP	Download protocol
IF protocol for DLAT antibody 13426-1-AP	Download protocol
IP protocol for DLAT antibody 13426-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell Res Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation Authors - Yunzi Mao View Article
mouse	WB	Nat Cell Biol RIP3 targets pyruvate dehydrogenase complex to increase aerobic respiration in TNF-induced necroptosis Authors - View Article
human,mouse	WB,IF,CoIP	Mol Cell Acetyl-CoA production by Mediator-bound 2-ketoacid dehydrogenases boosts de novo histone acetylation and is regulated by nitric oxide Authors - Marta Russo View Article
mouse	WB,IF	Adv Healthc Mater Biomimetic Nano-regulator that Induces Cuproptosis and Lactate-Depletion Mediated ROS Storm for Metalloimmunotherapy of Clear Cell Renal Cell Carcinoma Authors - Xiaotong Xu View Article
mouse	WB,IF	Adv Healthc Mater Copper-Based Composites Nanoparticles Improve Triple-Negative Breast Cancer Treatment with Induction of Apoptosis-Cuproptosis and Immune Activation Authors - Ning Wang View Article
rat	WB,IF	Sci Total Environ TRIM24-DTNBP1-ATP7A mediated astrocyte cuproptosis in cognition and memory dysfunction caused by Y2O3 NPs Authors - Ziwei Chen View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 13426-1-AP

WB analysis of HeLa using 13426-1-AP

WB analysis using 13426-1-AP

WB analysis of HAP1 using 13426-1-AP

IP experiment of HepG2 using 13426-1-AP

IHC staining of human liver cancer using 13426-1-AP

IHC staining of human liver cancer using 13426-1-AP

IHC staining of mouse kidney using 13426-1-AP

IHC staining of mouse kidney using 13426-1-AP

IHC staining of human colon cancer using 13426-1-AP

IF Staining of HepG2 using 13426-1-AP

IF Staining of HepG2 using 13426-1-AP

DLAT Polyclonal antibody

DLAT Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation

RIP3 targets pyruvate dehydrogenase complex to increase aerobic respiration in TNF-induced necroptosis

Acetyl-CoA production by Mediator-bound 2-ketoacid dehydrogenases boosts de novo histone acetylation and is regulated by nitric oxide

Biomimetic Nano-regulator that Induces Cuproptosis and Lactate-Depletion Mediated ROS Storm for Metalloimmunotherapy of Clear Cell Renal Cell Carcinoma

Copper-Based Composites Nanoparticles Improve Triple-Negative Breast Cancer Treatment with Induction of Apoptosis-Cuproptosis and Immune Activation

TRIM24-DTNBP1-ATP7A mediated astrocyte cuproptosis in cognition and memory dysfunction caused by Y2O3 NPs