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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DLAT Monoclonal antibody, PBS Only

DLAT Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F2B9

Cat no : 68303-1-PBS

Synonyms

PDC-E2, ODP2, EC:2.3.1.12, DLTA, Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DLAT Monoclonal antibody (68303-1-Ig)

    WB analysis using 68303-1-Ig (same clone as 68303-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68303-1-Ig (DLAT antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68303-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DLAT Monoclonal antibody (68303-1-Ig)

    IHC staining of human liver cancer using 68303-1-Ig (same clone as 68303-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68303-1-Ig (DLAT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68303-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DLAT Monoclonal antibody (68303-1-Ig)

    IHC staining of human liver cancer using 68303-1-Ig (same clone as 68303-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68303-1-Ig (DLAT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68303-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using DLAT Monoclonal antibody (68303-1-Ig)

    IHC staining of mouse kidney using 68303-1-Ig (same clone as 68303-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68303-1-Ig (DLAT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68303-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using DLAT Monoclonal antibody (68303-1-Ig)

    IHC staining of mouse kidney using 68303-1-Ig (same clone as 68303-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68303-1-Ig (DLAT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68303-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DLAT Monoclonal antibody (68303-1-Ig)

    IF Staining of HepG2 using 68303-1-Ig (same clone as 68303-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DLAT antibody (68303-1-Ig, Clone: 1F2B9 ) at dilution of 1:500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68303-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DLAT Monoclonal antibody (68303-1-Ig)

    IF Staining of HepG2 using 68303-1-Ig (same clone as 68303-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DLAT antibody (68303-1-Ig, Clone: 1F2B9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68303-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68303-1-PBS targets DLAT in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen DLAT fusion protein Ag4226 相同性解析による交差性が予測される生物種
Full Name dihydrolipoamide S-acetyltransferase
Calculated molecular weight 647 aa, 70 kDa
Observed molecular weight 69-70 kDa
GenBank accession numberBC039084
Gene symbol DLAT
Gene ID (NCBI) 1737
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.