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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DLC1 Polyclonal antibody

DLC1 Polyclonal Antibody for IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15460-1-AP

Synonyms

HP protein, HP, DLC-1, DLC 1, deleted in liver cancer 1

Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of mouse lung tissue using DLC1 Polyclonal antibody (15460-1-AP)

    IP experiment of mouse lung using 15460-1-AP

    IP result of anti-DLC1 (IP:15460-1-AP, 4ug; Detection:15460-1-AP 1:300) with mouse lung tissue lysate 2400ug.

  • Immunohistochemistry (IHC) staining of human spleen tissue using DLC1 Polyclonal antibody (15460-1-AP)

    IHC staining of human spleen using 15460-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 15460-1-AP (DLC1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using DLC1 Polyclonal antibody (15460-1-AP)

    IHC staining of human spleen using 15460-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 15460-1-AP (DLC1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using DLC1 Polyclonal antibody (15460-1-AP)

    IHC staining of human liver using 15460-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15460-1-AP (DLC1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using DLC1 Polyclonal antibody (15460-1-AP)

    IHC staining of human liver using 15460-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15460-1-AP (DLC1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DLC1 Polyclonal antibody (15460-1-AP)

    IF Staining of HepG2 using 15460-1-AP

    Immunofluorescent analysis of HepG2 cells, using DLC1 antibody 15460-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive IP detected inmouse lung tissue
Positive IHC detected inhuman spleen tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15460-1-AP targets DLC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DLC1 fusion protein Ag7732 相同性解析による交差性が予測される生物種
Full Name deleted in liver cancer 1
Calculated molecular weight 171 kDa
GenBank accession numberBC054511
Gene symbol DLC1
Gene ID (NCBI) 10395
RRIDAB_2246077
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for DLC1 antibody 15460-1-APDownload protocol
IF protocol for DLC1 antibody 15460-1-APDownload protocol
IP protocol for DLC1 antibody 15460-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biomaterials

Stiffness assisted cell-matrix remodeling trigger 3D mechanotransduction regulatory programs

Authors - Anna L Kersey
View Article