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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using DLD Monoclonal antibody (67702-1-Ig)

    WB analysis of HeLa using 67702-1-Ig

    WB result of DLD antibody (67702-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DLD transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using DLD Monoclonal antibody (67702-1-Ig)

    WB analysis using 67702-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67702-1-Ig (DLD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using DLD Monoclonal antibody (67702-1-Ig)

    WB analysis of NIH/3T3 using 67702-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67702-1-Ig (DLD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using DLD Monoclonal antibody (67702-1-Ig)

    WB analysis of HSC-T6 using 67702-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67702-1-Ig (DLD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DLD Monoclonal antibody (67702-1-Ig)

    IHC staining of human liver cancer using 67702-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67702-1-Ig (DLD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DLD Monoclonal antibody (67702-1-Ig)

    IHC staining of human liver cancer using 67702-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67702-1-Ig (DLD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  • Featured Product
  • KD/KO Validated

DLD Monoclonal antibody

DLD Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67702-1-Ig
Clone No.2E7G5
Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Synonyms

dihydrolipoamide dehydrogenase, DLD, DLDH, E3, GCSL, LAD, PHE3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration:  150 μL, 1100 ug/ml
20 μL, 1100 ug/ml
150 μL, 1100 ug/ml

¥67,000/ 150 μL

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHuH-7 cells, NIH/3T3 cells, HSC-T6 cells, HeLa cells, L02 cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

67702-1-Ig targets DLD in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DLD fusion protein Ag9680 相同性解析による交差性が予測される生物種
Full Name dihydrolipoamide dehydrogenase
Calculated molecular weight 509 aa, 54 kDa
Observed molecular weight54 kDa
GenBank accession numberBC018696
Gene Symbol DLD
Gene ID (NCBI) 1738
RRIDAB_2882894
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP09622
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

DLD(Dihydrolipoyl dehydrogenase, mitochondrial) is also named as GCSL, LAD, PHE3 and belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. It catalyzes the oxidation of dihydrolipoamide, hE3 uses two molecules : non-covalently bound FAD and a transiently bound substrate, NAD+. DLD is involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.

Protocols

Product Specific Protocols
WB protocol for DLD antibody 67702-1-IgDownload protocol
IHC protocol for DLD antibody 67702-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Immunol

The signature of cuproptosis-related immune genes predicts the tumor microenvironment and prognosis of prostate adenocarcinoma

Authors - Kai Yao
View Article