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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DLK1 Monoclonal antibody (67923-1-Ig)

    WB analysis using 67923-1-Ig (same clone as 67923-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67923-1-Ig (DLK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67923-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using DLK1 Monoclonal antibody (67923-1-Ig)

    WB analysis of human placenta using 67923-1-Ig (same clone as 67923-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67923-1-Ig (DLK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67923-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig adrenal gland tissue using DLK1 Monoclonal antibody (67923-1-Ig)

    WB analysis of pig adrenal gland using 67923-1-Ig (same clone as 67923-1-PBS)

    pig adrenal gland tissue were subjected to SDS PAGE followed by western blot with 67923-1-Ig (DLK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67923-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using DLK1 Monoclonal antibody (67923-1-Ig)

    IHC staining of human pancreas cancer using 67923-1-Ig (same clone as 67923-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67923-1-Ig (DLK1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67923-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using DLK1 Monoclonal antibody (67923-1-Ig)

    IHC staining of human pancreas cancer using 67923-1-Ig (same clone as 67923-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67923-1-Ig (DLK1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67923-1-PBS in a different storage buffer formulation.

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DLK1 Monoclonal antibody, PBS Only

DLK1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67923-1-PBS
Clone No.2C3H8

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, Indirect ELISA

Synonyms

pG2, FA1, DLK-1, DLK 1, DLK

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67923-1-PBS targets DLK1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DLK1 fusion protein Ag30916 相同性解析による交差性が予測される生物種
Full Name delta-like 1 homolog (Drosophila)
Calculated molecular weight 41 kDa
Observed molecular weight45-60 kDa
GenBank accession numberBC007741
Gene Symbol DLK1
Gene ID (NCBI) 8788
RRIDAB_2918675
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP80370
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

DLK1, also named PREF1, FA1, or pG2, is a transmembrane protein belonging to the epidermal growth factor (EGF)-like superfamily (PMID: 8095043). It contains six EGF-like repeats in the extracellular region. DLK1 is abundant in preadipocytes and regulate adipocyte differentiation negatively (PMID: 8500166). Deficiency of DLK1 gives rise to growth retardation and accelerated adiposity in mouse model. Expression of DLK1 is found in tumors with neuroendocrine features that implies DLK1 may be involved in neuroendocrine differentiation (PMID: 8095043). It has been reported overexpression of DLK1 could lead to the development of metabolic abnormalities by impairment of adipocyte function in mice (PMID: 12588883). The gene of DLK1 maps to chromosome 14q32, and encodes a 383-amino acid protein with a calculated molecular mass of 41 kDa. In preadipocytes, multiple discrete forms of DLK1 protein of 45-60 kDa are present, owing in part to N-linked glycosylation (PMID: 8500166).