- Featured Product
- KD/KO Validated
DLL4 Polyclonal antibody
DLL4 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF-P, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 21584-1-AP
Synonyms
Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | HUVEC cells, mouse brain tissue, A431 cells, bEnd.3 cells, mouse lung tissue |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human stomach cancer tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 15 publications below |
| IHC | See 3 publications below |
| IF | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
21584-1-AP targets DLL4 in WB, IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DLL4 fusion protein Ag16140 相同性解析による交差性が予測される生物種 |
| Full Name | delta-like 4 (Drosophila) |
| Calculated molecular weight | 685 aa, 75 kDa |
| Observed molecular weight | 70-80 kDa |
| GenBank accession number | BC106950 |
| Gene symbol | DLL4 |
| Gene ID (NCBI) | 54567 |
| RRID | AB_2878888 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DLL4, also named as Delta4, plays a role in the Notch signaling pathway. It activates Notch-1 and Notch-4. Notch signaling is activated upon engagement of the Notch receptor with its ligands, the DSL (Delta, Serrate, Lag2) proteins of single-pass type I membrane proteins. DLL4 is highly expressed in the vascular endothelium and is involved in angiogenesis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DLL4 antibody 21584-1-AP | Download protocol |
| IHC protocol for DLL4 antibody 21584-1-AP | Download protocol |
| IF protocol for DLL4 antibody 21584-1-AP | Download protocol |
| IP protocol for DLL4 antibody 21584-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells. | ||
Cell Rep Med Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application | ||
Angiogenesis Angiogenic responses in a 3D micro-engineered environment of primary endothelial cells and pericytes. | ||
Cancer Lett LRP1 induces anti-PD-1 resistance by modulating the DLL4-NOTCH2-CCL2 axis and redirecting M2-like macrophage polarisation in bladder cancer | ||
Bioact Mater Electrochemically derived nanographene oxide activates endothelial tip cells and promotes angiogenesis by binding endogenous lysophosphatidic acid. | ||
Theranostics Endothelial PERK-ATF4-JAG1 axis activated by T-ALL remodels bone marrow vascular niche. |
