DLX3 Polyclonal antibody

DLX3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 13261-3-AP

Synonyms

AI4, distal less homeobox 3, DLX3, Homeobox protein DLX 3, TDO


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Tested Applications

Positive WB detected inJAR cells, mouse lung tissue, NCCIT cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13261-3-AP targets DLX3 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DLX3 fusion protein Ag3865 相同性解析による交差性が予測される生物種
Full Name distal-less homeobox 3
Calculated molecular weight 287 aa, 32 kDa
Observed molecular weight 32-38 kDa
GenBank accession numberBC028970
Gene symbol DLX3
Gene ID (NCBI) 1747
RRIDAB_2292996
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Distal-less homeobox 3 (DLX3), a member of the Dlx family, plays a crucial role in epithelium, hair, bone, tooth and placental development. DLX3 is expressed during the calcium (Ca2+)-dependent epidermal differentiation process. The calculated molecular weight of DLX3 is 32 kDa. This antibody detects the 32-38 kDa protein in SDS-PAGE. (PMID: 31029881)

Protocols

Product Specific Protocols
WB protocol for DLX3 antibody 13261-3-APDownload protocol
IHC protocol for DLX3 antibody 13261-3-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP,CoIP,IF

J Biol Chem

New insights into the regulation of placental growth factor gene expression by the transcription factors GCM1 and DLX3 in human placenta.

Authors - Yueh-Ho Chiu
mouseWB,CoIP

Stem Cells

IPO7 promotes odontoblastic differentiation and inhibits osteoblastic differentiation through regulation of RUNX2 expression and translocation

Authors - Yue Zhang