DLX3 Polyclonal antibody
DLX3 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 13261-3-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | JAR cells, mouse lung tissue, NCCIT cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
| CoIP | See 2 publications below |
Product Information
13261-3-AP targets DLX3 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DLX3 fusion protein Ag3865 相同性解析による交差性が予測される生物種 |
| Full Name | distal-less homeobox 3 |
| Calculated molecular weight | 287 aa, 32 kDa |
| Observed molecular weight | 32-38 kDa |
| GenBank accession number | BC028970 |
| Gene symbol | DLX3 |
| Gene ID (NCBI) | 1747 |
| RRID | AB_2292996 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Distal-less homeobox 3 (DLX3), a member of the Dlx family, plays a crucial role in epithelium, hair, bone, tooth and placental development. DLX3 is expressed during the calcium (Ca2+)-dependent epidermal differentiation process. The calculated molecular weight of DLX3 is 32 kDa. This antibody detects the 32-38 kDa protein in SDS-PAGE. (PMID: 31029881)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DLX3 antibody 13261-3-AP | Download protocol |
| IHC protocol for DLX3 antibody 13261-3-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Biol Chem New insights into the regulation of placental growth factor gene expression by the transcription factors GCM1 and DLX3 in human placenta. | ||
Stem Cells IPO7 promotes odontoblastic differentiation and inhibits osteoblastic differentiation through regulation of RUNX2 expression and translocation | ||
Exp Mol Med METTL3-mediated pre-miR-665/DLX3 m6A methylation facilitates the committed differentiation of stem cells from apical papilla
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