DLX3 Polyclonal antibody
DLX3 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, IF, IHC, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 13261-3-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | JAR cells, mouse lung tissue, NCCIT cells |
Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 2 publications below |
IF | See 1 publications below |
IP | See 1 publications below |
CoIP | See 2 publications below |
Product Information
13261-3-AP targets DLX3 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | DLX3 fusion protein Ag3865 相同性解析による交差性が予測される生物種 |
Full Name | distal-less homeobox 3 |
Calculated molecular weight | 287 aa, 32 kDa |
Observed molecular weight | 32-38 kDa |
GenBank accession number | BC028970 |
Gene symbol | DLX3 |
Gene ID (NCBI) | 1747 |
RRID | AB_2292996 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Distal-less homeobox 3 (DLX3), a member of the Dlx family, plays a crucial role in epithelium, hair, bone, tooth and placental development. DLX3 is expressed during the calcium (Ca2+)-dependent epidermal differentiation process. The calculated molecular weight of DLX3 is 32 kDa. This antibody detects the 32-38 kDa protein in SDS-PAGE. (PMID: 31029881)
Protocols
Product Specific Protocols | |
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WB protocol for DLX3 antibody 13261-3-AP | Download protocol |
IHC protocol for DLX3 antibody 13261-3-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Biol Chem New insights into the regulation of placental growth factor gene expression by the transcription factors GCM1 and DLX3 in human placenta. | ||
Stem Cells IPO7 promotes odontoblastic differentiation and inhibits osteoblastic differentiation through regulation of RUNX2 expression and translocation |