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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DLX5 Monoclonal antibody

DLX5 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C9G1

Cat no : 67111-1-Ig

Synonyms

distal less homeobox 5, DLX5, Homeobox protein DLX 5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DLX5 Monoclonal antibody (67111-1-Ig)

    WB analysis using 67111-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67111-1-Ig (DLX5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using DLX5 Monoclonal antibody (67111-1-Ig)

    WB analysis of A375 using 67111-1-Ig

    A375 cells were subjected to SDS PAGE followed by western blot with 67111-1-Ig (DLX5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-251 cells using DLX5 Monoclonal antibody (67111-1-Ig)

    WB analysis of U-251 using 67111-1-Ig

    U-251 cells were subjected to SDS PAGE followed by western blot with 67111-1-Ig (DLX5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of THP-1 cells using DLX5 Monoclonal antibody (67111-1-Ig)

    WB analysis of THP-1 using 67111-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 67111-1-Ig (DLX5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DLX5 Monoclonal antibody (67111-1-Ig)

    IHC staining of mouse brain using 67111-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67111-1-Ig (DLX5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DLX5 Monoclonal antibody (67111-1-Ig)

    IHC staining of mouse brain using 67111-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67111-1-Ig (DLX5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DLX5 Monoclonal antibody (67111-1-Ig)

    IHC staining of mouse brain using 67111-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67111-1-Ig (DLX5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DLX5 Monoclonal antibody (67111-1-Ig)

    IHC staining of mouse brain using 67111-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67111-1-Ig (DLX5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inPC-12 cells, A375 cells, U-251 cells, THP-1 cells, pig brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67111-1-Ig targets DLX5 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DLX5 fusion protein Ag17040 相同性解析による交差性が予測される生物種
Full Name distal-less homeobox 5
Calculated molecular weight 32 kDa
Observed molecular weight 33-38 kDa
GenBank accession numberBC006226
Gene symbol DLX5
Gene ID (NCBI) 1749
RRIDAB_2882415
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

DLX5 belongs to the DLX family of homeobox transcription factors (Dlx1, Dlx2, Dlx5 and Dlx6), which is expressed in overlapping domains at different stages of cell differentiation in the subpallium and controls differentiation of GABAergic neurons. DLX5 is an immediate early BMP-responsive transcriptional activator that essential for osteoblast differentiation. It can bind to and stimulate the homeodomain-response element of the ALPL and SP7 promoter. DLX5 can form homodimer with DLX2(~34kd) and heterodimer with MSX2(~29kd).

Protocols

Product Specific Protocols
WB protocol for DLX5 antibody 67111-1-IgDownload protocol
IHC protocol for DLX5 antibody 67111-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols