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  • Featured Product
  • KD/KO Validated

DLX6 Polyclonal antibody

DLX6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 23216-1-AP

Synonyms

distal less homeobox 6, DLX6

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of NCCIT cells using DLX6 Polyclonal antibody (23216-1-AP)

    WB analysis of NCCIT using 23216-1-AP

    NCCIT cell were subjected to SDS PAGE followed by western blot with 23216-1-AP (DLX6 Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using DLX6 Polyclonal antibody (23216-1-AP)

    IHC staining of mouse embryo using 23216-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 23216-1-AP (DLX6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using DLX6 Polyclonal antibody (23216-1-AP)

    IHC staining of mouse embryo using 23216-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 23216-1-AP (DLX6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using DLX6 Polyclonal antibody (23216-1-AP)

    IHC staining of mouse embryo using 23216-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 23216-1-AP (DLX6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inNCCIT cell
Positive IHC detected inmouse embryo tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23216-1-AP targets DLX6 in WB, IF, IHC, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DLX6 fusion protein Ag19724 相同性解析による交差性が予測される生物種
Full Name distal-less homeobox 6
Calculated molecular weight 175 aa, 20 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC069363
Gene symbol DLX6
Gene ID (NCBI) 1750
RRIDAB_2879233
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DLX6 antibody 23216-1-APDownload protocol
IHC protocol for DLX6 antibody 23216-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncol Rep

DANCR promotes glioma cell autophagy and proliferation via the miR‑33b/DLX6/ATG7 axis

Authors - Wei Yu
  • KD Validated
View Article
humanChIP,IF

Nat Commun

The transcriptional regulatory network modulating human trophoblast stem cells to extravillous trophoblast differentiation

Authors - Mijeong Kim
View Article