Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse testis tissue, HeLa cells, human testis tissue, rat testis tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)-P | IF-P : 1:600-1:2400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
| IF | See 10 publications below |
Product Information
13714-1-AP targets DMC1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DMC1 fusion protein Ag4653 相同性解析による交差性が予測される生物種 |
| Full Name | DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination (yeast) |
| Calculated molecular weight | 165 aa, 18 kDa, 38 kDa |
| Observed molecular weight | 37 kDa |
| GenBank accession number | BC035658 |
| Gene Symbol | DMC1 |
| Gene ID (NCBI) | 11144 |
| RRID | AB_2091076 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14565 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DMC1 antibody 13714-1-AP | Download protocol |
| IHC protocol for DMC1 antibody 13714-1-AP | Download protocol |
| IF protocol for DMC1 antibody 13714-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res NAT10-mediated N4-acetylcytidine modification is required for meiosis entry and progression in male germ cells. | ||
Nucleic Acids Res MEIOK21: a new component of meiotic recombination bridges required for spermatogenesis. | ||
Cell Prolif ZFP541 and KCTD19 regulate chromatin organization and transcription programs for male meiotic progression | ||
Cell Death Differ NBS1 is required for SPO11-linked DNA double-strand break repair in male meiosis. | ||
Elife A missense in HSF2BP causing primary ovarian insufficiency affects meiotic recombination by its novel interactor C19ORF57/BRME1. | ||
Front Oncol The Ectopic Expression of Meiosis Regulatory Genes in Cutaneous T-Cell Lymphomas (CTCL). |