DMC1 Polyclonal antibody

Name: DMC1 Polyclonal antibody
Brand: Proteintech
SKU: 13714-1-AP

DMC1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 13714-1-AP

Synonyms

LIM15, HsLim15, DMC1H

Validation Data Gallery

Filter:

AllWBIHCIF-PIF/ICC

WB analysis using 13714-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13714-1-AP (DMC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 13714-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13714-1-AP (DMC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 13714-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 13714-1-AP (DMC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of mouse testis using 13714-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13714-1-AP (DMC1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 13714-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13714-1-AP (DMC1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse testis using 13714-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using DMC1 antibody (13714-1-AP) at dilution of 1:1200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 13714-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DMC1 antibody (13714-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Cat No. 13714-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse testis tissue, HeLa cells, human testis tissue, rat testis tissue
Positive IHC detected in	mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse testis tissue
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:2000-1:8000
Immunofluorescence (IF)-P	IF-P : 1:600-1:2400
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IHC	See 1 publications below
IF	See 10 publications below

Product Information

13714-1-AP targets DMC1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DMC1 fusion protein Ag4653 相同性解析による交差性が予測される生物種
Full Name	DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination (yeast)
Calculated molecular weight	165 aa, 18 kDa, 38 kDa
Observed molecular weight	37 kDa
GenBank accession number	BC035658
Gene symbol	DMC1
Gene ID (NCBI)	11144
RRID	AB_2091076
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for DMC1 antibody 13714-1-AP	Download protocol
IHC protocol for DMC1 antibody 13714-1-AP	Download protocol
IF protocol for DMC1 antibody 13714-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Nucleic Acids Res NAT10-mediated N4-acetylcytidine modification is required for meiosis entry and progression in male germ cells. Authors - Lu Chen View Article
mouse	IF	Nucleic Acids Res MEIOK21: a new component of meiotic recombination bridges required for spermatogenesis. Authors - Yongliang Shang View Article
		Cell Prolif ZFP541 and KCTD19 regulate chromatin organization and transcription programs for male meiotic progression Authors - Xu Zhou View Article
mouse	IF	Cell Death Differ NBS1 is required for SPO11-linked DNA double-strand break repair in male meiosis. Authors - Bin Zhang View Article
mouse	IF	Elife A missense in HSF2BP causing primary ovarian insufficiency affects meiotic recombination by its novel interactor C19ORF57/BRME1. Authors - Natalia Felipe-Medina View Article
human	IHC	Front Oncol The Ectopic Expression of Meiosis Regulatory Genes in Cutaneous T-Cell Lymphomas (CTCL). Authors - Jennifer Gantchev View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DMC1 Polyclonal antibody

DMC1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Validation Data Gallery

WB analysis using 13714-1-AP

WB analysis of HeLa using 13714-1-AP

WB analysis of human testis using 13714-1-AP

IHC staining of mouse testis using 13714-1-AP

IHC staining of mouse testis using 13714-1-AP

IF Staining of mouse testis using 13714-1-AP

IF Staining of HeLa using 13714-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

NAT10-mediated N4-acetylcytidine modification is required for meiosis entry and progression in male germ cells.

MEIOK21: a new component of meiotic recombination bridges required for spermatogenesis.

ZFP541 and KCTD19 regulate chromatin organization and transcription programs for male meiotic progression

NBS1 is required for SPO11-linked DNA double-strand break repair in male meiosis.

A missense in HSF2BP causing primary ovarian insufficiency affects meiotic recombination by its novel interactor C19ORF57/BRME1.

The Ectopic Expression of Meiosis Regulatory Genes in Cutaneous T-Cell Lymphomas (CTCL).