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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DMGDH Polyclonal antibody, PBS Only

DMGDH Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 24813-1-PBS

Synonyms

ME2GLYDH, M2GD, EC:1.5.8.4, DMGDHD, dimethylglycine dehydrogenase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DMGDH Polyclonal antibody (24813-1-AP)

    WB analysis using 24813-1-AP (same clone as 24813-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24813-1-AP (DMGDH antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24813-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using DMGDH Polyclonal antibody (24813-1-AP)

    WB analysis of mouse liver using 24813-1-AP (same clone as 24813-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 24813-1-AP (DMGDH Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24813-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using DMGDH Polyclonal antibody (24813-1-AP)

    WB analysis of rat liver using 24813-1-AP (same clone as 24813-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 24813-1-AP (DMGDH Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24813-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DMGDH Polyclonal antibody (24813-1-AP)

    IHC staining of human liver cancer using 24813-1-AP (same clone as 24813-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24813-1-AP (DMGDH Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 24813-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DMGDH Polyclonal antibody (24813-1-AP)

    IHC staining of human liver cancer using 24813-1-AP (same clone as 24813-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24813-1-AP (DMGDH Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 24813-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DMGDH Polyclonal antibody (24813-1-AP)

    IF Staining of HepG2 using 24813-1-AP (same clone as 24813-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using DMGDH antibody (24813-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24813-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using DMGDH Polyclonal antibody (24813-1-AP)

    FC experiment of HepG2 using 24813-1-AP (same clone as 24813-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug DMGDH Polyclonal antibody (24813-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 24813-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24813-1-PBS targets DMGDH in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DMGDH fusion protein Ag20529 相同性解析による交差性が予測される生物種
Full Name dimethylglycine dehydrogenase
Calculated molecular weight 866 aa, 97 kDa
Observed molecular weight 97 kDa
GenBank accession numberBC022388
Gene symbol DMGDH
Gene ID (NCBI) 29958
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

DMGDH(Dimethylglycine dehydrogenase, mitochondrial) is also named as ME2GLYDH and belongs to the GcvT family. It catalyzes the oxidative demethylation of dimethylglycine to form sarcosine and it plays a role in choline catabolism in the liver. The full length protein has a transit peptide.