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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DMRT1 Polyclonal antibody

DMRT1 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

Cat no : 14313-1-AP

Synonyms

Doublesex- and mab-3-related transcription factor 1, DMT1, CT154

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DMRT1 Polyclonal antibody (14313-1-AP)

    WB analysis using 14313-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14313-1-AP (DMRT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using DMRT1 Polyclonal antibody (14313-1-AP)

    IP experiment of mouse testis using 14313-1-AP

    IP result of anti-DMRT1 (IP:14313-1-AP, 4ug; Detection:14313-1-AP 1:500) with mouse testis tissue lysate 2400 ug.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DMRT1 Polyclonal antibody (14313-1-AP)

    IHC staining of mouse testis using 14313-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14313-1-AP (DMRT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DMRT1 Polyclonal antibody (14313-1-AP)

    IHC staining of mouse testis using 14313-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14313-1-AP (DMRT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using DMRT1 Polyclonal antibody (14313-1-AP)

    IHC staining of human testis using 14313-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14313-1-AP (DMRT1 Antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using DMRT1 Polyclonal antibody (14313-1-AP)

    IHC staining of human testis using 14313-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14313-1-AP (DMRT1 Antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using DMRT1 Polyclonal antibody (14313-1-AP)

    IF Staining of mouse testis using 14313-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using DMRT1 antibody (14313-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using DMRT1 Polyclonal antibody (14313-1-AP)

    IF Staining of mouse testis using 14313-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using DMRT1 antibody (14313-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inmouse testis tissue, human testis
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14313-1-AP targets DMRT1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DMRT1 fusion protein Ag5547 相同性解析による交差性が予測される生物種
Full Name doublesex and mab-3 related transcription factor 1
Calculated molecular weight 39 kDa
Observed molecular weight39 kDa
GenBank accession numberBC040847
Gene symbol DMRT1
Gene ID (NCBI) 1761
RRIDAB_2918035
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DMRT1, also named DMT1, belongs to the DMRT family. DMRT1 is one of the most conserved transcription factors in a variety of species and is involved in sex determination and germ cell differentiation. DMRT1 prevents the sexual switch from male to female, represses pluripotency, and balances mitosis versus meiosis. DMRT1 also maintains spermatogenesis and replenishes mGSCs by collaborating with Plzf and Sal-like protein 4 (PMID: 33420764). DMRT1 has 3 isoforms with the molecular mass of 18, 29, and 39 kDa.

Protocols

Product Specific Protocols
WB protocol for DMRT1 antibody 14313-1-APDownload protocol
IHC protocol for DMRT1 antibody 14313-1-APDownload protocol
IF protocol for DMRT1 antibody 14313-1-APDownload protocol
IP protocol for DMRT1 antibody 14313-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols