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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DMRT1 Polyclonal antibody, PBS Only

DMRT1 Polyclonal Antibody for WB, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28149-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using DMRT1 Polyclonal antibody (28149-1-AP)

    WB analysis using 28149-1-AP (same clone as 28149-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28149-1-AP (DMRT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28149-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using DMRT1 Polyclonal antibody (28149-1-AP)

    IF Staining of mouse testis using 28149-1-AP (same clone as 28149-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using DMRT1 antibody (28149-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28149-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28149-1-PBS targets DMRT1 in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DMRT1 fusion protein Ag28006 相同性解析による交差性が予測される生物種
Full Name doublesex and mab-3 related transcription factor 1
Calculated molecular weight 39 kDa
Observed molecular weight39 kDa
GenBank accession numberBC040847
Gene symbol DMRT1
Gene ID (NCBI) 1761
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

DMRT1, also named DMT1, belongs to the DMRT family. DMRT1 is one of the most conserved transcription factors in a variety of species and is involved in sex determination and germ cell differentiation. DMRT1 prevents the sexual switch from male to female, represses pluripotency, and balances mitosis versus meiosis. DMRT1 also maintains spermatogenesis and replenishes mGSCs by collaborating with Plzf and Sal-like protein 4 (PMID: 33420764). DMRT1 has 3 isoforms with the molecular mass of 18, 29, and 39 kDa.