DMXL2 Polyclonal antibody
DMXL2 Polyclonal Antibody for IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 24415-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human cerebellum tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
The immunogen of 24415-1-AP is DMXL2 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DMXL2 fusion protein Ag19720 相同性解析による交差性が予測される生物種 |
| Full Name | Dmx-like 2 |
| Calculated molecular weight | 3036 aa, 340 kDa |
| GenBank accession number | BC140781 |
| Gene symbol | DMXL2 |
| Gene ID (NCBI) | 23312 |
| RRID | AB_2879534 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DMXL2, also named as rabconnectin-3, is a functional regulator of mammalian notch signaling with WD domains. Rabconnectin-3 is abundantly expressed in the brain where it is enriched in the synaptic vesicle fraction. Immunofluorescence and immunoelectron microscopy revealed that rabconnectin-3 was concentrated on synaptic vesicles at synapses. It may serve as a scaffold molecule for both Rab3 GEP and GAP on synaptic vesicles. In western blotting, we got 340 kDa and 104 kDa in mouse tissues. The two bands may represent different isoforms.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for DMXL2 antibody 24415-1-AP | Download protocol |
| IP protocol for DMXL2 antibody 24415-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Brain Biallelic DMXL2 mutations impair autophagy and cause Ohtahara syndrome with progressive course. | ||
Oncotarget DMXL2 drives epithelial to mesenchymal transition in hormonal therapy resistant breast cancer through Notch hyper-activation. |
