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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DNAH2 Polyclonal antibody

DNAH2 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 18841-1-AP

Synonyms

Ciliary dynein heavy chain 2, DNAH2, DNAHC2, DNHD3, Dynein heavy chain 2, axonemal, KIAA1503

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of human colon tissue using DNAH2 Polyclonal antibody (18841-1-AP)

    IHC staining of human colon using 18841-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18841-1-AP (DNAH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using DNAH2 Polyclonal antibody (18841-1-AP)

    IHC staining of human colon using 18841-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18841-1-AP (DNAH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using DNAH2 Polyclonal antibody (18841-1-AP)

    IHC staining of human lung using 18841-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 18841-1-AP (DNAH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using DNAH2 Polyclonal antibody (18841-1-AP)

    IHC staining of human lung using 18841-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 18841-1-AP (DNAH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using DNAH2 Polyclonal antibody (18841-1-AP)

    IF Staining of U2OS using 18841-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using 18841-1-AP (DNAH2 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive IHC detected inhuman colon tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 2 publications below

Product Information

18841-1-AP targets DNAH2 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name dynein, axonemal, heavy chain 2
Calculated molecular weight 508 kDa
GenBank accession numberNM_020877
Gene symbol DNAH2
Gene ID (NCBI) 146754
RRIDAB_2878565
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DNAH2, also named as DNAHC2, DNHD3 and KIAA1503, belongs to the dynein heavy chain family. DNAH2 force generating protein of respiratory cilia and produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. DNAH2 involved in sperm motility; implicated in sperm flagellar assembly.

Protocols

Product Specific Protocols
IHC protocol for DNAH2 antibody 18841-1-APDownload protocol
IF protocol for DNAH2 antibody 18841-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Andrology

A novel homozygous LRRC6 mutation causes male infertility with asthenozoospermia and primary ciliary dyskinesia in humans

Authors - Shengjia Shi
View Article
humanIF

Asian J Androl

A novel homozygous mutation of CFAP300 identified in a Chinese patient with primary ciliary dyskinesia and infertility

Authors - Zheng Zhou
View Article