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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human lung tissue using DNAH5 Polyclonal antibody (31079-1-AP)

    IHC staining of human lung using 31079-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 31079-1-AP (DNAH5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using DNAH5 Polyclonal antibody (31079-1-AP)

    IHC staining of mouse lung using 31079-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 31079-1-AP (DNAH5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DNAH5 Polyclonal antibody (31079-1-AP)

    IF Staining of HeLa using 31079-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DNAH5 antibody (31079-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DNAH5 Polyclonal antibody (31079-1-AP)

    IF Staining of HepG2 using 31079-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DNAH5 antibody (31079-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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DNAH5 Polyclonal antibody

DNAH5 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 31079-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Synonyms

KIAA1603, HL1, Dynein heavy chain 5, axonemal, DNAHC5, Ciliary dynein heavy chain 5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman lung tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31079-1-AP targets DNAH5 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAH5 fusion protein Ag34647 相同性解析による交差性が予測される生物種
Full Name dynein, axonemal, heavy chain 5
Calculated molecular weight529 kDa
GenBank accession numberNM_001369
Gene Symbol DNAH5
Gene ID (NCBI) 1767
RRIDAB_3669844
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ8TE73
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Dynein axonemal heavy chain 5 (DNAH5) is an axonemal heavy chain dynein, part of a microtubule-associated motor protein complex. DNAH5 is a large gene comprising 79 exons and one alternative first exon and encodes a heavy chain of the ODA. We have recently shown that recessive DNAH5 mutations are responsible for PCD characterized by ODA defects. DNAH5 mutations cause PCD and randomization of left-right asymmetry (PMID: 15750039).

Protocols

Product Specific Protocols
IHC protocol for DNAH5 antibody 31079-1-APDownload protocol
IF protocol for DNAH5 antibody 31079-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols