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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DNAH6 Polyclonal antibody

DNAH6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30073-1-AP

Synonyms

Ciliary dynein heavy chain 6, DKFZp667K087, DNAH6, Dnahc6, DNHL1, Dynein heavy chain 6, axonemal, FLJ23529, FLJ37357, HL 2, HL2, KIAA1697

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DNAH6 Polyclonal antibody (30073-1-AP)

    WB analysis using 30073-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 30073-1-AP (DNAH6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DNAH6 Polyclonal antibody (30073-1-AP)

    IHC staining of mouse testis using 30073-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30073-1-AP (DNAH6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DNAH6 Polyclonal antibody (30073-1-AP)

    IHC staining of mouse testis using 30073-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30073-1-AP (DNAH6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using DNAH6 Polyclonal antibody (30073-1-AP)

    IF Staining of hTERT-RPE1 using 30073-1-AP

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using DNAH6 antibody (30073-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using DNAH6 Polyclonal antibody (30073-1-AP)

    IF Staining of hTERT-RPE1 using 30073-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using DNAH6 antibody (30073-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite® Plus 488 ARL13B antibody (CL488-17711, green).

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Tested Applications

Positive WB detected inmouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30073-1-AP targets DNAH6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAH6 fusion protein Ag32364 相同性解析による交差性が予測される生物種
Full Name dynein, axonemal, heavy chain 6
Calculated molecular weight475 kDa
Observed molecular weight 475 kDa
GenBank accession numberBC104884
Gene symbol DNAH6
Gene ID (NCBI) 1768
RRIDAB_2935510
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DNAH6(Dynein heavy chain 6, axonemal) is also named as DNAHC6, DNHL1, HL2, KIAA1697 and belongs to the dynein heavy chain family.Dyneins are microtubule-associated motor protein complexes composed of several heavy, light, and intermediate chains(PMID:8812413). It is a force generating protein of respiratory cilia and produces force towards the minus ends of microtubules.This antibody is specific to DNAH6.

Protocols

Product Specific Protocols
WB protocol for DNAH6 antibody 30073-1-APDownload protocol
IHC protocol for DNAH6 antibody 30073-1-APDownload protocol
IF protocol for DNAH6 antibody 30073-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols