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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DNAI1 Polyclonal antibody

DNAI1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12756-1-AP

Synonyms

PCD, ICS1, Dynein axonemal intermediate chain 1, CILD1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human brain tissue using DNAI1 Polyclonal antibody (12756-1-AP)

    WB analysis of human brain using 12756-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12756-1-AP (DNAI1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using DNAI1 Polyclonal antibody (12756-1-AP)

    WB analysis using 12756-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12756-1-AP (DNAI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using DNAI1 Polyclonal antibody (12756-1-AP)

    WB analysis of human brain using 12756-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12756-1-AP (DNAI1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using DNAI1 Polyclonal antibody (12756-1-AP)

    IF Staining of hTERT-RPE1 using 12756-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using DNAI1 antibody (12756-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red).

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Tested Applications

Positive WB detected inmouse testis tissue, human brain tissue, rat testis tissue
Positive IF/ICC detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12756-1-AP targets DNAI1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAI1 fusion protein Ag3473 相同性解析による交差性が予測される生物種
Full Name dynein, axonemal, intermediate chain 1
Calculated molecular weight 699 aa, 79 kDa
Observed molecular weight 80-85 kDa
GenBank accession numberBC030583
Gene symbol DNAI1
Gene ID (NCBI) 27019
RRIDAB_10643244
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DNAI1 antibody 12756-1-APDownload protocol
IF protocol for DNAI1 antibody 12756-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Cell Biol

Pih1d3 is required for cytoplasmic preassembly of axonemal dynein in mouse sperm.

Authors - Fenglan Dong
View Article
mouseWB,IF

EBioMedicine

CFAP70 is a solid and valuable target for the genetic diagnosis of oligo-astheno-teratozoospermia in infertile men

Authors - Hui-Juan Jin
View Article