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DNAJA1 Monoclonal antibody

DNAJA1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgM

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B10A10

Cat no : 67184-1-Ig

Synonyms

DJ 2, DjA1, DnaJ protein homolog 2, DNAJ2, DNAJA1, hDJ 2, HDJ2, Heat shock 40 kDa protein 4, Heat shock protein J2, HSDJ, HSJ 2, HSJ2, HSPF4, Human DnaJ protein 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DNAJA1 Monoclonal antibody (67184-1-Ig)

    WB analysis using 67184-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67184-1-Ig (DNAJA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DNAJA1 Monoclonal antibody (67184-1-Ig)

    WB analysis of HeLa using 67184-1-Ig

    WB result of DNAJA1 antibody (67184-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNAJA1 transfected HeLa cells.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DNAJA1 Monoclonal antibody (67184-1-Ig)

    IF Staining of HepG2 using 67184-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DNAJA1 antibody (67184-1-Ig, Clone: 1B10A10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inA549 cells, HeLa cells, MCF-7 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67184-1-Ig targets DNAJA1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen DNAJA1 fusion protein Ag2284 相同性解析による交差性が予測される生物種
Full Name DnaJ (Hsp40) homolog, subfamily A, member 1
Calculated molecular weight 397 aa, 45 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC008182
Gene symbol DNAJA1
Gene ID (NCBI) 3301
RRIDAB_2882480
Conjugate Unconjugated
Form Liquid
Purification MethodEuglobulin precipitation
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DNAJA1 is a member of the DnaJ proteins (also known as Hsp40 or Hsc40) which are cochaperones to DnaK (Hsp70) and function to modulate protein assembly, disassembly, and translocation. DNAJA1 has been reported to get involved in various processes, including virus infection, tau clearance, and mutant p53 degradation.

Protocols

Product Specific Protocols
WB protocol for DNAJA1 antibody 67184-1-IgDownload protocol
IF protocol for DNAJA1 antibody 67184-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols