DNAJA2 Polyclonal antibody

Name: DNAJA2 Polyclonal antibody
Brand: Proteintech
SKU: 12236-1-AP

DNAJA2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12236-1-AP

Synonyms

HIRA interacting protein 4, Dnj3, DNAJ, DJA2, Dj3

Validation Data Gallery

Filter:

AllWBIPIF/ICC

WB analysis of A375 using 12236-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 12236-1-AP (DNAJA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 12236-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12236-1-AP (DNAJA2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 12236-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12236-1-AP (DNAJA2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
IP experiment of A375 using 12236-1-AP
IP result of anti-DNAJA2 (IP:12236-1-AP, 4ug; Detection:12236-1-AP 1:300) with A375 cells lysate 3600 ug.
IF Staining of MCF-7 using 12236-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 12236-1-AP (DNAJA2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 12236-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A375 cells, mouse kidney tissue, human brain tissue
Positive IP detected in	A375 cells
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 8 publications below
IF	See 2 publications below
IP	See 2 publications below
CoIP	See 1 publications below

Product Information

12236-1-AP targets DNAJA2 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DNAJA2 fusion protein Ag2877 相同性解析による交差性が予測される生物種
Full Name	DnaJ (Hsp40) homolog, subfamily A, member 2
Calculated molecular weight	417 aa, 46 kDa
Observed molecular weight	46 kDa
GenBank accession number	BC015809
Gene symbol	DNAJA2
Gene ID (NCBI)	10294
RRID	AB_2230709
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for DNAJA2 antibody 12236-1-AP	Download protocol
IF protocol for DNAJA2 antibody 12236-1-AP	Download protocol
IP protocol for DNAJA2 antibody 12236-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun Metamorphism in TDP-43 prion-like domain determines chaperone recognition Authors - Jaime Carrasco View Article
human	WB	Int J Cancer Down-regulation of BiP/GRP78 sensitizes resistant prostate cancer cells to gene-therapeutic overexpression of REIC/Dkk-3. Authors - Tanimoto Ryuta R View Article
human	WB	J Biol Chem A cytosolic chaperone complex controls folding and degradation of type III CD38. Authors - Yang Wu View Article
human	WB	Cancers (Basel) Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin Authors - Mohamed Alalem View Article
human,mouse	WB,IP	J Biol Chem Quantitative interactome proteomics identifies a proteostasis network for GABAA receptors Authors - Ya-Juan Wang View Article
mouse	WB	Cancer Res N-myc-Mediated Translation Control Is a Therapeutic Vulnerability in Medulloblastoma Authors - Duygu Kuzuoglu-Ozturk View Article

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研究分野

Target

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Species Reactivity

Species Reactivity

Conjugate

Conjugate

DNAJA2 Polyclonal antibody

DNAJA2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of A375 using 12236-1-AP

WB analysis of human brain using 12236-1-AP

WB analysis of mouse kidney using 12236-1-AP

IP experiment of A375 using 12236-1-AP

IF Staining of MCF-7 using 12236-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Metamorphism in TDP-43 prion-like domain determines chaperone recognition

Down-regulation of BiP/GRP78 sensitizes resistant prostate cancer cells to gene-therapeutic overexpression of REIC/Dkk-3.

A cytosolic chaperone complex controls folding and degradation of type III CD38.

Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin

Quantitative interactome proteomics identifies a proteostasis network for GABAA receptors

N-myc-Mediated Translation Control Is a Therapeutic Vulnerability in Medulloblastoma