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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DNAJB1 Monoclonal antibody, PBS Only

DNAJB1 Monoclonal Antibody for Indirect ELISA, WB

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

1E8E10

Cat no : 67422-1-PBS

Synonyms

hDj-1, Hdj1, hDj 1, DNAJ1, DnaJ protein homolog 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DNAJB1 Monoclonal antibody (67422-1-Ig)

    WB analysis using 67422-1-Ig (same clone as 67422-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67422-1-Ig (DNAJB1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67422-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using DNAJB1 Monoclonal antibody (67422-1-Ig)

    FC experiment of HeLa using 67422-1-Ig (same clone as 67422-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human DNAJB1 (67422-1-Ig, Clone:1E8E10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67422-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67422-1-PBS targets DNAJB1 in Indirect ELISA, WB applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DNAJB1 fusion protein Ag3830 相同性解析による交差性が予測される生物種
Full Name DnaJ (Hsp40) homolog, subfamily B, member 1
Calculated molecular weight 340 aa, 40 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC019827
Gene symbol DNAJB1
Gene ID (NCBI) 3337
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

DNAJB1 is a member of the heat shock 40 protein family and has been involved in a variety of cellular processes, including the proteasome pathway, endoplasmic reticulum stress and viral infection. DNAJB1-PRKACA gene fusions have been considered diagnostic for fibrolamellar hepatocellular carcinoma.