Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, Jurkat cells, C2C12 cells, C6 cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82676-1-RR targets DNAJB1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | DNAJB1 fusion protein Ag3830 相同性解析による交差性が予測される生物種 |
| Full Name | DnaJ (Hsp40) homolog, subfamily B, member 1 |
| Calculated molecular weight | 340 aa, 40 kDa |
| Observed molecular weight | 37-40 kDa |
| GenBank accession number | BC019827 |
| Gene Symbol | DNAJB1 |
| Gene ID (NCBI) | 3337 |
| RRID | AB_3086502 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P25685 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DNAJB1 is a member of the heat shock 40 protein family and has been involved in a variety of cellular processes, including the proteasome pathway, endoplasmic reticulum stress and viral infection. DNAJB1-PRKACA gene fusions have been considered diagnostic for fibrolamellar hepatocellular carcinoma.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DNAJB1 antibody 82676-1-RR | Download protocol |
| IHC protocol for DNAJB1 antibody 82676-1-RR | Download protocol |
| IF protocol for DNAJB1 antibody 82676-1-RR | Download protocol |
| IP protocol for DNAJB1 antibody 82676-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |