Featured Product
KD/KO Validated

DNAJB11 Polyclonal antibody

Name: DNAJB11 Polyclonal antibody
Brand: Proteintech
SKU: 15484-1-AP

DNAJB11 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15484-1-AP

Synonyms

ABBP 2, ABBP2, APOBEC1 binding protein 2, DJ9, DnaJ protein homolog 9, DNAJB11, EDJ, ER associated DNAJ, ER associated dnaJ protein 3, ERdj3, ERj3, ERj3p, hDj 9, hDj9, HEDJ, Human DnaJ protein 9, PRO1080, PWP1 interacting protein 4, UNQ537, UNQ537/PRO1080

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 15484-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15484-1-AP (DNAJB11 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 15484-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15484-1-AP (DNAJB11 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 15484-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 15484-1-AP (DNAJB11 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse heart using 15484-1-AP
IP result of anti-DNAJB11 (IP:15484-1-AP, 4ug; Detection:15484-1-AP 1:500) with mouse heart tissue lysate 3200ug.
IHC staining of human ovary tumor using 15484-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15484-1-AP (DNAJB11 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human ovary tumor using 15484-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15484-1-AP (DNAJB11 antibody) at dilution of 1:100 (under 10x lens).
IF Staining of HepG2 using 15484-1-AP
Immunofluorescent analysis of HepG2 cells, using DNAJB11 antibody 15484-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Cat No. 15484-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, mouse liver tissue, mouse heart tissue, HepG2 cells, rat liver tissue
Positive IP detected in	mouse heart tissue
Positive IHC detected in	human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 19 publications below
IF	See 1 publications below
IP	See 2 publications below

Product Information

15484-1-AP targets DNAJB11 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DNAJB11 fusion protein Ag7832 相同性解析による交差性が予測される生物種
Full Name	DnaJ (Hsp40) homolog, subfamily B, member 11
Calculated molecular weight	41 kDa
Observed molecular weight	40 kDa
GenBank accession number	BC001144
Gene symbol	DNAJB11
Gene ID (NCBI)	51726
RRID	AB_2094400
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for DNAJB11 antibody 15484-1-AP	Download protocol
IHC protocol for DNAJB11 antibody 15484-1-AP	Download protocol
IF protocol for DNAJB11 antibody 15484-1-AP	Download protocol
IP protocol for DNAJB11 antibody 15484-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Science The endoplasmic reticulum P5A-ATPase is a transmembrane helix dislocase. Authors - Michael J McKenna View Article
human	WB	Cell Defining Hsp70 Subnetworks in Dengue Virus Replication Reveals Key Vulnerability in Flavivirus Infection. Authors - Shuhei Taguwa KD Validated View Article
mouse	WB	Mol Cell Differentiation Drives Widespread Rewiring of the Neural Stem Cell Chaperone Network. Authors - Willianne I M Vonk View Article
human	WB, IP	EMBO J Unfolded protein response-induced ERdj3 secretion links ER stress to extracellular proteostasis. Authors - Joseph C Genereux KD Validated View Article
human	WB	Cell Chem Biol Quantitative Interactome Proteomics Reveals a Molecular Basis for ATF6-Dependent Regulation of a Destabilized Amyloidogenic Protein. Authors - Lars Plate View Article
human	WB	Cell Death Dis Shutdown of ER-associated degradation pathway rescues functions of mutant iduronate 2-sulfatase linked to mucopolysaccharidosis type II. Authors - Yosuke Osaki KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DNAJB11 Polyclonal antibody

DNAJB11 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 15484-1-AP

WB analysis of mouse liver using 15484-1-AP

WB analysis of mouse heart using 15484-1-AP

IP experiment of mouse heart using 15484-1-AP

IHC staining of human ovary tumor using 15484-1-AP

IHC staining of human ovary tumor using 15484-1-AP

IF Staining of HepG2 using 15484-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

The endoplasmic reticulum P5A-ATPase is a transmembrane helix dislocase.

Defining Hsp70 Subnetworks in Dengue Virus Replication Reveals Key Vulnerability in Flavivirus Infection.

Differentiation Drives Widespread Rewiring of the Neural Stem Cell Chaperone Network.

Unfolded protein response-induced ERdj3 secretion links ER stress to extracellular proteostasis.

Quantitative Interactome Proteomics Reveals a Molecular Basis for ATF6-Dependent Regulation of a Destabilized Amyloidogenic Protein.

Shutdown of ER-associated degradation pathway rescues functions of mutant iduronate 2-sulfatase linked to mucopolysaccharidosis type II.