DNAJB5 Polyclonal antibody
DNAJB5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 16453-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | rat heart tissue, HeLa cells, HepG2 cells |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human heart tissue, human kidney tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
16453-1-AP targets DNAJB5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | DNAJB5 fusion protein Ag9341 相同性解析による交差性が予測される生物種 |
Full Name | DnaJ (Hsp40) homolog, subfamily B, member 5 |
Calculated molecular weight | 386 aa, 44 kDa |
Observed molecular weight | 39 kDa |
GenBank accession number | BC012115 |
Gene symbol | DNAJB5 |
Gene ID (NCBI) | 25822 |
RRID | AB_2094724 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DNAJB5, also named as Hsc40, is an Hsp40 Family Protein with MW 39-40 kDa. It mediates antihypertrophic effects of Thioredoxin1 in the heart. This protein has some isoforms with MW 39 kDa and 47-51 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for DNAJB5 antibody 16453-1-AP | Download protocol |
IHC protocol for DNAJB5 antibody 16453-1-AP | Download protocol |
IF protocol for DNAJB5 antibody 16453-1-AP | Download protocol |
IP protocol for DNAJB5 antibody 16453-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |