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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using DNAJB6 Monoclonal antibody (66587-1-Ig)

    WB analysis of HEK-293 using 66587-1-Ig (same clone as 66587-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66587-1-Ig (DNAJB6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66587-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using DNAJB6 Monoclonal antibody (66587-1-Ig)

    WB analysis of Jurkat using 66587-1-Ig (same clone as 66587-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66587-1-Ig (DNAJB6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66587-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using DNAJB6 Monoclonal antibody (66587-1-Ig)

    WB analysis of mouse brain using 66587-1-Ig (same clone as 66587-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66587-1-Ig (DNAJB6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66587-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using DNAJB6 Monoclonal antibody (66587-1-Ig)

    WB analysis of HeLa using 66587-1-Ig (same clone as 66587-1-PBS)

    WB result of DNAJB6 antibody (66587-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNAJB6 transfected HeLa cells. This data was developed using the same antibody clone with 66587-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using DNAJB6 Monoclonal antibody (66587-1-Ig)

    WB analysis of HSC-T6 using 66587-1-Ig (same clone as 66587-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66587-1-Ig (DNAJB6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66587-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Neuro-2a cells using DNAJB6 Monoclonal antibody (66587-1-Ig)

    WB analysis of Neuro-2a using 66587-1-Ig (same clone as 66587-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66587-1-Ig (DNAJB6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66587-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using DNAJB6 Monoclonal antibody (66587-1-Ig)

    WB analysis of pig brain using 66587-1-Ig (same clone as 66587-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66587-1-Ig (DNAJB6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66587-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using DNAJB6 Monoclonal antibody (66587-1-Ig)

    WB analysis of rat brain using 66587-1-Ig (same clone as 66587-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66587-1-Ig (DNAJB6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66587-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using DNAJB6 Monoclonal antibody (66587-1-Ig)

    IHC staining of human testis using 66587-1-Ig (same clone as 66587-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66587-1-Ig (DNAJB6 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66587-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using DNAJB6 Monoclonal antibody (66587-1-Ig)

    IHC staining of human testis using 66587-1-Ig (same clone as 66587-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66587-1-Ig (DNAJB6 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66587-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DNAJB6 Monoclonal antibody (66587-1-Ig)

    IHC staining of mouse brain using 66587-1-Ig (same clone as 66587-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66587-1-Ig (DNAJB6 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66587-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DNAJB6 Monoclonal antibody (66587-1-Ig)

    IHC staining of mouse brain using 66587-1-Ig (same clone as 66587-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66587-1-Ig (DNAJB6 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66587-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DNAJB6 Monoclonal antibody (66587-1-Ig)

    IHC staining of mouse brain using 66587-1-Ig (same clone as 66587-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66587-1-Ig (DNAJB6 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66587-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DNAJB6 Monoclonal antibody (66587-1-Ig)

    IHC staining of mouse brain using 66587-1-Ig (same clone as 66587-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66587-1-Ig (DNAJB6 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66587-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

DNAJB6 Monoclonal antibody, PBS Only

DNAJB6 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66587-1-PBS
Clone No.1A1D2

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, Indirect ELISA

Synonyms

DJ4, DnaJ, DNAJB6, Heat shock protein J2, HHDJ1, HSJ 2, HSJ2, MRJ, MSJ 1, MSJ1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66587-1-PBS targets DNAJB6 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DNAJB6 fusion protein Ag2258 相同性解析による交差性が予測される生物種
Full Name DnaJ (Hsp40) homolog, subfamily B, member 6
Calculated molecular weight 241 aa, 27 kDa
Observed molecular weight 27 kDa, 38 kDa
GenBank accession numberBC000177
Gene Symbol DNAJB6
Gene ID (NCBI) 10049
RRIDAB_2881947
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO75190
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

DNAJB6, belongs to the heat shock protein 40 (HSP40) family, a noncanonical member of the DNAJ-chaperone family, is mainly expressed in neurons. It plays an important role in recovery from misfolded protein aggregates (PMID: 27276715). As seen by the western blot stained for DNAJB6, there are 2 splice forms of the protein resulting in a longer isoform (38 kDa) and a shorter one (27 kDa). DNAJB6 (27 kDa) isoform but not DNAJB6 (38 kDa) isoform is effective with suppressing cytoplasmic protein aggregation (PMID: 20159555). The antibody 66587-1-Ig recognizes both isoforms.