Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse kidney tissue, mouse testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human breast cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IHC | See 3 publications below |
Product Information
13157-1-AP targets DNAJB9 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DNAJB9 fusion protein Ag3789 相同性解析による交差性が予測される生物種 |
| Full Name | DnaJ (Hsp40) homolog, subfamily B, member 9 |
| Calculated molecular weight | 223 aa, 26 kDa |
| Observed molecular weight | 26-30 kDa |
| GenBank accession number | BC028912 |
| Gene Symbol | DNAJB9 |
| Gene ID (NCBI) | 4189 |
| RRID | AB_2877918 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UBS3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DNAJB9 antibody 13157-1-AP | Download protocol |
| IHC protocol for DNAJB9 antibody 13157-1-AP | Download protocol |
| IP protocol for DNAJB9 antibody 13157-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Pineal Res Melatonin alleviates PM2.5 -induced glucose metabolism disorder and lipidome alteration by regulating endoplasmic reticulum stress | ||
Mol Cell Coordination between Two Branches of the Unfolded Protein Response Determines Apoptotic Cell Fate. | ||
Elife Cell-based HTS identifies a chemical chaperone for preventing ER protein aggregation and proteotoxicity. | ||
Hum Mol Genet XBP1 variant 1 (Xv1) promotes mitosis of cancer cells involving upregulation of the polyglutamylase TTLL6. | ||
Pathol Int Early onset Congo red-positive fibrillary glomerulonephritis associated with glomerular DNAJB9 deposits mimicking renal amyloidosis | ||
Case Rep Nephrol Fibrillary Glomerulopathy with a High Level of Myeloperoxidase-ANCA: A Case Report. |