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Target

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Species Reactivity

Species Reactivity

Conjugate

Conjugate

DNAJC1 Polyclonal antibody

DNAJC1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12362-1-AP

Synonyms

DnaJ protein homolog MTJ1, DNAJC1, DNAJL1, ERdj1, HTJ1, MTJ1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DNAJC1 Polyclonal antibody (12362-1-AP)

    WB analysis using 12362-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12362-1-AP (DNAJC1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using DNAJC1 Polyclonal antibody (12362-1-AP)

    WB analysis of A549 using 12362-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 12362-1-AP (DNAJC1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Raji cells using DNAJC1 Polyclonal antibody (12362-1-AP)

    IP experiment of Raji using 12362-1-AP

    IP result of anti-DNAJC1 (IP:12362-1-AP, 4ug; Detection:12362-1-AP 1:1000) with Raji cells lysate 3200 ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DNAJC1 Polyclonal antibody (12362-1-AP)

    IHC staining of human breast cancer using 12362-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12362-1-AP (DNAJC1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DNAJC1 Polyclonal antibody (12362-1-AP)

    IHC staining of human breast cancer using 12362-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12362-1-AP (DNAJC1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DNAJC1 Polyclonal antibody (12362-1-AP)

    IHC staining of human liver cancer using 12362-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12362-1-AP (DNAJC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DNAJC1 Polyclonal antibody (12362-1-AP)

    IHC staining of human liver cancer using 12362-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12362-1-AP (DNAJC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inMCF-7 cells, A549 cells, Raji cells, NIH/3T3 cells
Positive IP detected inRaji cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12362-1-AP targets DNAJC1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAJC1 fusion protein Ag3030 相同性解析による交差性が予測される生物種
Full Name DnaJ (Hsp40) homolog, subfamily C, member 1
Calculated molecular weight 554 aa, 64 kDa
Observed molecular weight 64 kDa
GenBank accession numberBC030955
Gene symbol DNAJC1
Gene ID (NCBI) 64215
RRIDAB_2094754
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DNAJC1 antibody 12362-1-APDownload protocol
IHC protocol for DNAJC1 antibody 12362-1-APDownload protocol
IP protocol for DNAJC1 antibody 12362-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cancer Res Clin Oncol

DNAJC1 facilitates glioblastoma progression by promoting extracellular matrix reorganization and macrophage infiltration

Authors - Han Zhang
View Article