Validation Data Gallery
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Tested Applications
| Positive WB detected in | A549 cells, BxPC-3 cells, HEK-293 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 4 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
Product Information
12338-1-AP targets DNAJC12 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DNAJC12 fusion protein Ag2989 相同性解析による交差性が予測される生物種 |
| Full Name | DnaJ (Hsp40) homolog, subfamily C, member 12 |
| Calculated molecular weight | 198 aa, 23 kDa |
| Observed molecular weight | 28 kDa |
| GenBank accession number | BC017018 |
| Gene Symbol | DNAJC12 |
| Gene ID (NCBI) | 56521 |
| RRID | AB_2246188 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UKB3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DNAJC12 antibody 12338-1-AP | Download protocol |
| IHC protocol for DNAJC12 antibody 12338-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Rep Med Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application | ||
Cell Stress Chaperones The co-chaperone DNAJC12 binds to Hsc70 and is upregulated by endoplasmic reticulum stress. | ||
J Mol Cell Biol DNAJC12 downregulation induces neuroblastoma progression via increased histone H4K5 lactylation
| ||
Ann Surg Oncol Increased Expression of DNAJC12 is Associated with Aggressive Phenotype of Gastric Cancer.
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