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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DNAJC13 Polyclonal antibody (31492-1-AP)

    WB analysis using 31492-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31492-1-AP (DNAJC13 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DNAJC13 Polyclonal antibody (31492-1-AP)

    IF Staining of HeLa using 31492-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DNAJC13 antibody (31492-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

DNAJC13 Polyclonal antibody

DNAJC13 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31492-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

RME8, RME 8, KIAA0678

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31492-1-AP targets DNAJC13 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAJC13 fusion protein Ag35412 相同性解析による交差性が予測される生物種
Full Name DnaJ (Hsp40) homolog, subfamily C, member 13
Observed molecular weight254 kDa
GenBank accession numberBC043583
Gene Symbol DNAJC13
Gene ID (NCBI) 23317
RRIDAB_3670005
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDO75165
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DNAJC13 is a large protein with 2,243 amino acids. Due to the presence of the central DNAJ domain, DNAJC13 binds HSC70 (heat shock cognate 70) and acts as a co-chaperone in mediating HSC70 cellular functions. Within the cell, DNAJC13 is primarily localized at membranous structures. This interaction is mediated, at least in part, by the binding to phosphoinositides (PI) through its N-terminal sequence. RME-8/DNAJC13 is involved in several endosomal functions such as protein sorting, endosomal tubulation, transport processes including the retrograde transport from the endosome to the trans-Golgi network (TGN), the formation of endosomal degradative microdomains, and the recycling of membrane receptors. (PMID: 32322926)

Protocols

Product Specific Protocols
WB protocol for DNAJC13 antibody 31492-1-APDownload protocol
IF protocol for DNAJC13 antibody 31492-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols