DNAJC2/MPP11 Polyclonal antibody

DNAJC2/MPP11 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11971-1-AP

Synonyms

DNAJC2, DnaJ homolog subfamily C member 2, N-terminally processed, ZRF1, MPP11, MPHOSPH11


☆対象製品が30%オフ!組換えタンパク質&免染用試薬 >>> キャンペーン実施中!


Tested Applications

Positive WB detected inCaco-2 cells, HEK-293 cells, HeLa cells, HepG2 cells, K-562 cells, mouse liver tissue, PC-3 cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

11971-1-AP targets DNAJC2/MPP11 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAJC2/MPP11 fusion protein Ag2594 相同性解析による交差性が予測される生物種
Full Name DnaJ (Hsp40) homolog, subfamily C, member 2
Calculated molecular weight 72 kDa
Observed molecular weight 66-75 kDa
GenBank accession numberBC056682
Gene symbol DNAJC2
Gene ID (NCBI) 27000
RRIDAB_10695893
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DNAJC2/MPP11 antibody 11971-1-APDownload protocol
IHC protocol for DNAJC2/MPP11 antibody 11971-1-APDownload protocol
IF protocol for DNAJC2/MPP11 antibody 11971-1-APDownload protocol
IP protocol for DNAJC2/MPP11 antibody 11971-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO J

Dual roles of HSP70 chaperone HSPA1 in quality control of nascent and newly synthesized proteins.

Authors - Guiyou Tian
humanWB

Cancers (Basel)

Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin

Authors - Mohamed Alalem