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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DNAJC21 Monoclonal antibody

DNAJC21 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2F8A9

Cat no : 68343-1-Ig

Synonyms

DNAJA5, DNAJC21, GS3, JJJ1, Protein GS3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DNAJC21 Monoclonal antibody (68343-1-Ig)

    WB analysis using 68343-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68343-1-Ig (DNAJC21 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using DNAJC21 Monoclonal antibody (68343-1-Ig)

    IF Staining of U2OS using 68343-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using DNAJC21 antibody (68343-1-Ig, Clone: 2F8A9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inK-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68343-1-Ig targets DNAJC21 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DNAJC21 fusion protein Ag20003 相同性解析による交差性が予測される生物種
Full Name DnaJ (Hsp40) homolog, subfamily C, member 21
Calculated molecular weight 531 aa, 58 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC107577
Gene symbol DNAJC21
Gene ID (NCBI) 134218
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DNAJC21 is a ubiquitously expressed DnaJ (heat shock protein 40) chaperone protein. Recently DNAJC21 was reported to be implicated in rRNA biogenesis. Loss of DNAJC21 induced abnormal cell morphology and inhibited cell growth. Mutations in DNAJC21 cause bone marrow failure syndrome. (27346687)

Protocols

Product Specific Protocols
WB protocol for DNAJC21 antibody 68343-1-IgDownload protocol
IF protocol for DNAJC21 antibody 68343-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols