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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DNAJC4 Polyclonal antibody

DNAJC4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13853-1-AP

Synonyms

DANJC4, DnaJ like protein HSPF2, DNAJC4, HSPF2, MCG18

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using DNAJC4 Polyclonal antibody (13853-1-AP)

    WB analysis of MCF-7 using 13853-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 13853-1-AP (DNAJC4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DNAJC4 Polyclonal antibody (13853-1-AP)

    WB analysis of HeLa using 13853-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13853-1-AP (DNAJC4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using DNAJC4 Polyclonal antibody (13853-1-AP)

    WB analysis of HepG2 using 13853-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13853-1-AP (DNAJC4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DNAJC4 Polyclonal antibody (13853-1-AP)

    IHC staining of human breast cancer using 13853-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 13853-1-AP (DNAJC4 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DNAJC4 Polyclonal antibody (13853-1-AP)

    IHC staining of human breast cancer using 13853-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 13853-1-AP (DNAJC4 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13853-1-AP targets DNAJC4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAJC4 fusion protein Ag4826 相同性解析による交差性が予測される生物種
Full Name DnaJ (Hsp40) homolog, subfamily C, member 4
Calculated molecular weight 28 kDa
Observed molecular weight 28-30 kDa
GenBank accession numberBC044584
Gene symbol DNAJC4
Gene ID (NCBI) 3338
RRIDAB_2095224
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DNAJC4 antibody 13853-1-APDownload protocol
IHC protocol for DNAJC4 antibody 13853-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols