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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using DNAJC5B Polyclonal antibody (17364-1-AP)

    WB analysis of mouse testis using 17364-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 17364-1-AP (DNAJC5B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using DNAJC5B Polyclonal antibody (17364-1-AP)

    IP experiment of mouse testis using 17364-1-AP

    IP result of anti-DNAJC5B (IP:17364-1-AP, 3ug; Detection:17364-1-AP 1:1500) with mouse testis tissue lysate 4000ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DNAJC5B Polyclonal antibody (17364-1-AP)

    IF Staining of HepG2 using 17364-1-AP

    Immunofluorescent analysis of HepG2 cells, using DNAJC5B antibody 17364-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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DNAJC5B Polyclonal antibody

DNAJC5B Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 17364-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Synonyms

Cysteine-string protein isoform beta, CSP-beta, CSP beta, Beta cysteine string protein, Beta CSP

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse testis tissue
Positive IP detected inmouse testis tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17364-1-AP targets DNAJC5B in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAJC5B fusion protein Ag10744 相同性解析による交差性が予測される生物種
Full Name DnaJ (Hsp40) homolog, subfamily C, member 5 beta
Calculated molecular weight 199 aa, 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC016742
Gene Symbol DNAJC5B
Gene ID (NCBI) 85479
RRIDAB_2095062
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UF47
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DNAJC5B encodes cysteine string proteins (CSP) beta. CSPs belong to the DnaJ-like chaperone family and play an important role in regulated exocytosis in neurons and endocrine cells. Mammals express three CSP proteins, α, β, γ. In contrast to the CSPα which is ubiquitously expressed, CSPβ is restricted to testis and auditory hair cell neurons. The predicted MW of DNAJC5B is around 22 kDa, while some modified forms exist (PMID: 17034881)

Protocols

Product Specific Protocols
WB protocol for DNAJC5B antibody 17364-1-APDownload protocol
IF protocol for DNAJC5B antibody 17364-1-APDownload protocol
IP protocol for DNAJC5B antibody 17364-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols