DNAJC6/AUXILIN Polyclonal antibody
DNAJC6/AUXILIN Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 21941-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, HeLa cells, human brain tissue, mouse cerebellum tissue, rat brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 1 publications below |
Product Information
The immunogen of 21941-1-AP is DNAJC6/AUXILIN Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | DNAJC6/AUXILIN fusion protein Ag16612 相同性解析による交差性が予測される生物種 |
Full Name | DnaJ (Hsp40) homolog, subfamily C, member 6 |
Calculated molecular weight | 970 aa, 106 kDa |
Observed molecular weight | 100-120 kDa, 86 kDa |
GenBank accession number | BC109279 |
Gene symbol | DNAJC6/AUXILIN |
Gene ID (NCBI) | 9829 |
RRID | AB_2878951 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DNAJC6, also named AUXILIN, belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. It is a neuronal protein that functions specifically in the pathway of clathrin-mediated endocytosis. It shares homology with GAK, and the two proteins act as cochaperones to support the HSC70 -dependent clathrin uncoating of clathrin-coated vesicles (PMID: 2016009). DNAJC6 is expressed in various brain regions, including cerebellum, corpus callosum, cortex, striatum, brainstem, pons, putamen, spinal cord and substantia nigra (PMID: 23211418).
Protocols
Product Specific Protocols | |
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WB protocol for DNAJC6/AUXILIN antibody 21941-1-AP | Download protocol |
IHC protocol for DNAJC6/AUXILIN antibody 21941-1-AP | Download protocol |
IF protocol for DNAJC6/AUXILIN antibody 21941-1-AP | Download protocol |
IP protocol for DNAJC6/AUXILIN antibody 21941-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Sci Rep Differential regulation of synaptic AP-2/clathrin vesicle uncoating in synaptic plasticity. | ||
Cancers (Basel) Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin |