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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DNAJC7 Recombinant antibody (85157-4-RR)

    WB analysis using 85157-4-RR (same clone as 85157-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85157-4-RR (DNAJC7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85157-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DNAJC7 Recombinant antibody (85157-4-RR)

    IHC staining of mouse brain using 85157-4-RR (same clone as 85157-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85157-4-RR (DNAJC7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85157-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DNAJC7 Recombinant antibody (85157-4-RR)

    IF Staining of HepG2 using 85157-4-RR (same clone as 85157-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DNAJC7 antibody (85157-4-RR, Clone: 242875B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85157-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using DNAJC7 Recombinant antibody (85157-4-RR)

    FC experiment of HeLa using 85157-4-RR (same clone as 85157-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug DNAJC7 Recombinant antibody (85157-4-RR, Clone:242875B3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 85157-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85157-4-RR

    Affinity and Kinetic Characterization of 85157-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85157-4-RR against Human DNAJC7 were performed. The affinity constant is 0.767 nM.

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DNAJC7 Recombinant antibody, PBS Only

DNAJC7 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 85157-4-PBS
Clone No.242875B3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

242875B3, CCRP, DANJC7, DNAJC 7, TPR2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85157-4-PBS targets DNAJC7 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DNAJC7 fusion protein Ag1572 相同性解析による交差性が予測される生物種
Full Name DnaJ (Hsp40) homolog, subfamily C, member 7
Calculated molecular weight 56 kDa
Observed molecular weight56 kDa
GenBank accession numberBC011837
Gene Symbol DNAJC7
Gene ID (NCBI) 7266
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99615
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

DnaJC7, otherwise referred to as Tpr2 or CCRP, is a conserved, type of J protein that is highly expressed within the brain. DnaJC7 has been found to modulate the flow of substrates from Hsp70 to Hsp90, providing a control mechanism in proteins such as glucocorticoids and progesterone receptor.