Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, human brain tissue, mouse brain tissue, rat brain tissue |
| Positive IP detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15809-1-AP targets DNAL1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DNAL1 fusion protein Ag8545 相同性解析による交差性が予測される生物種 |
| Full Name | dynein, axonemal, light chain 1 |
| Calculated molecular weight | 151 aa, 17 kDa |
| Observed molecular weight | 22 kDa |
| GenBank accession number | BC005343 |
| Gene Symbol | DNAL1 |
| Gene ID (NCBI) | 83544 |
| RRID | AB_2095365 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q4LDG9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DNAL1 was originally documented as the Chlamydomonas outer arm dynein axonemal light chain 1. DNAL1 has been documented to link components of the cytoskeleton with the molecular motor dynein, perhaps acting as a scaffold for larger functional structures (PMID: 22018492).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DNAL1 antibody 15809-1-AP | Download protocol |
| IP protocol for DNAL1 antibody 15809-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |