Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse testis tissue, rat testis tissue |
| Positive IP detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 8 publications below |
| IF | See 9 publications below |
| CoIP | See 1 publications below |
Product Information
17601-1-AP targets DNALI1 in WB, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DNALI1 fusion protein Ag11652 相同性解析による交差性が予測される生物種 |
| Full Name | dynein, axonemal, light intermediate chain 1 |
| Calculated molecular weight | 258 aa, 30 kDa |
| Observed molecular weight | 30-33 kDa |
| GenBank accession number | BC046117 |
| Gene Symbol | DNALI1 |
| Gene ID (NCBI) | 7802 |
| RRID | AB_2095372 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14645 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DNALI1 belongs to the inner dynein arm light chain family and encodes a subunit of the light intermediate chain of inner dynein arms of the axoneme of sperm flagella (PMID: 36792588). DNALI1 promotes neurodegeneration after traumatic brain injury via inhibition of autophagosome-lysosome fusion (PMID: 38348540). Mutation in DNALI1 Causes Male Infertility with Severe Oligoasthenoteratozoospermia in Humans (PMID: 38212584).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DNALI1 antibody 17601-1-AP | Download protocol |
| IP protocol for DNALI1 antibody 17601-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) DNALI1 Promotes Neurodegeneration after Traumatic Brain Injury via Inhibition of Autophagosome-Lysosome Fusion | ||
Elife DNAH3 deficiency causes flagellar inner dynein arm loss and male infertility in humans and mice | ||
Front Cell Dev Biol PIH1D3-knockout rats exhibit full ciliopathy features and dysfunctional pre-assembly and loading of dynein arms in motile cilia | ||
Development CCDC183 is essential for cytoplasmic invagination around the flagellum during spermiogenesis and male fertility | ||
Clin Genet DNAH2 is a novel candidate gene associated with multiple morphological abnormalities of the sperm flagella. | ||