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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DNase I Polyclonal antibody (16659-1-AP)

    WB analysis using 16659-1-AP

    human urine sample were subjected to SDS PAGE followed by western blot with 16659-1-AP (DNase I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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DNase I Polyclonal antibody

DNase I Polyclonal Antibody for WB, ELISA
Cat No. 16659-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

DNASE1, EC:3.1.21.1, DRNI, DNL1, Deoxyribonuclease-1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman urine sample

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16659-1-AP targets DNase I in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNase I fusion protein Ag10117 相同性解析による交差性が予測される生物種
Full Name deoxyribonuclease I
Calculated molecular weight 282 aa, 31 kDa
Observed molecular weight 31-40 kDa
GenBank accession numberBC029437
Gene Symbol DNASE1
Gene ID (NCBI) 1773
RRIDAB_3669253
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP24855
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Deoxyribonuclease I (DNase I), also known as DNL1 and DRNI, belongs to the DNase I family. DNase I is a DNA-digestive glycoprotein that cleaves both single- and double stranded DNA through hydrolysis of the phosphodiester bond. In humans, the DNase I enzyme is primarily secreted by the pancreas, parotid, and pituitary glands into the digestive system and bloodstream. Since DNase I is ubiquitously expressed in human tissues, it is thought to play a similar anti-autoimmune role in other organs as well (PMID: 21806320). The calculated molecular weight of DNase I is 31 kDa. Sometimes higher molecular weight around 40 kDa can also be observed, which is caused by glycosylation (PMID: 34329318).

Protocols

Product Specific Protocols
WB protocol for DNase I antibody 16659-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols