DNase I Recombinant antibody
DNase I Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
240222F11
Cat no : 83337-6-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse heart tissue, human urine sample |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of 83337-6-RR is DNase I Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | DNase I fusion protein Ag10117 相同性解析による交差性が予測される生物種 |
Full Name | deoxyribonuclease I |
Calculated molecular weight | 282 aa, 31 kDa |
Observed molecular weight | 31-40 kDa |
GenBank accession number | BC029437 |
Gene symbol | DNASE1 |
Gene ID (NCBI) | 1773 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Deoxyribonuclease I (DNase I), also known as DNL1 and DRNI, belongs to the DNase I family. DNase I is a DNA-digestive glycoprotein that cleaves both single- and double stranded DNA through hydrolysis of the phosphodiester bond. In humans, the DNase I enzyme is primarily secreted by the pancreas, parotid, and pituitary glands into the digestive system and bloodstream. Since DNase I is ubiquitously expressed in human tissues, it is thought to play a similar anti-autoimmune role in other organs as well (PMID: 21806320). The calculated molecular weight of DNase I is 31 kDa. Sometimes higher molecular weight around 40 kDa can also be observed, which is caused by glycosylation (PMID: 34329318).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for DNase I antibody 83337-6-RR | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |