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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

DNASE1L1 Polyclonal antibody

DNASE1L1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13653-1-AP

Synonyms

DNase I-like 1, DNase I like 1, DNAS1L1, Deoxyribonuclease-1-like 1, Deoxyribonuclease I-like 1

Validation Data Gallery

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  • Western Blot (WB) analysis of rat skeletal muscle tissue using DNASE1L1 Polyclonal antibody (13653-1-AP)

    WB analysis of rat skeletal muscle using 13653-1-AP

    rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13653-1-AP (DNASE1L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using DNASE1L1 Polyclonal antibody (13653-1-AP)

    WB analysis of mouse skeletal muscle using 13653-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13653-1-AP (DNASE1L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse skeletal muscle tissue using DNASE1L1 Polyclonal antibody (13653-1-AP)

    IP experiment of mouse skeletal muscle using 13653-1-AP

    IP result of anti-DNASE1L1 (IP:13653-1-AP, 4ug; Detection:13653-1-AP 1:800) with mouse skeletal muscle tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using DNASE1L1 Polyclonal antibody (13653-1-AP)

    IHC staining of human skeletal muscle using 13653-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 13653-1-AP (DNASE1L1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using DNASE1L1 Polyclonal antibody (13653-1-AP)

    IHC staining of human skeletal muscle using 13653-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 13653-1-AP (DNASE1L1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using DNASE1L1 Polyclonal antibody (13653-1-AP)

    IF Staining of MCF-7 using 13653-1-AP

    Immunofluorescent analysis of MCF-7 cells, using DNASE1L1 antibody 13653-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13653-1-AP targets DNASE1L1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNASE1L1 fusion protein Ag4222 相同性解析による交差性が予測される生物種
Full Name deoxyribonuclease I-like 1
Calculated molecular weight 302 aa, 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC028092
Gene symbol DNASE1L1
Gene ID (NCBI) 1774
RRIDAB_2230813
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DNASE1L1 antibody 13653-1-APDownload protocol
IHC protocol for DNASE1L1 antibody 13653-1-APDownload protocol
IF protocol for DNASE1L1 antibody 13653-1-APDownload protocol
IP protocol for DNASE1L1 antibody 13653-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Cells

New Findings: Hindlimb Unloading Causes Nucleocytoplasmic Ca2+ Overload and DNA Damage in Skeletal Muscle

Authors - Huajian Yang
View Article
ratWB

J Tradit Chin Med

Synergistic effect of schizandrin A and DNase I knockdown on high glucose induced beta cell apoptosis by decreasing intracellular calcium concentration

Authors - Zhu Bin
  • KD Validated
View Article