DNASE1L3 Polyclonal antibody
DNASE1L3 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 11330-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, Raji cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:200-1:600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
11330-1-AP targets DNASE1L3 in WB, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | DNASE1L3 fusion protein Ag1873 相同性解析による交差性が予測される生物種 |
Full Name | deoxyribonuclease I-like 3 |
Calculated molecular weight | 305 aa, 36 kDa |
Observed molecular weight | 33-38 kDa |
GenBank accession number | BC015831 |
Gene symbol | DNASE1L3 |
Gene ID (NCBI) | 1776 |
RRID | AB_2095527 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Deoxyribonuclease 1-like 3 (DNASE1L3, DNase gamma, DNase Y, LS-DNase) is member of a DNASE1 protein family that is identified by similar biochemical properties such as Ca2+/Mg2+ dependency and an optimal pH of about 7.0 as well as by a high similarity in their nucleic acid and amino acid sequences [PMID:15796714]. It is an endonuclease and cleaves double-stranded DNA (and single-stranded DNA) producing 3'-OH/5'-P ends, which is Ca2+/Mg2+-dependent and inhibited by Zn2+, but not by monomeric actin [PMID:9665719]. In addition, it has been suggested to be involved in apoptotic DNA fragmentation of thymocytes neuronal PC12 cells, C2C12 myoblasts and of cell lines expressing the recombinant nuclease [PMID: 7957253, 9307016]
Protocols
Product Specific Protocols | |
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WB protocol for DNASE1L3 antibody 11330-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |