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Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using DNASE1L3 Polyclonal antibody (11330-1-AP)

    WB analysis of Raji using 11330-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 11330-1-AP (DNASE1L3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using DNASE1L3 Polyclonal antibody (11330-1-AP)

    WB analysis of HEK-293 using 11330-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11330-1-AP (DNASE1L3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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DNASE1L3 Polyclonal antibody

DNASE1L3 Polyclonal Antibody for WB, ELISA
Cat No. 11330-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Deoxyribonuclease gamma, deoxyribonuclease I like 3, DHP2, DNAS1L3, DNase gamma, DNase I homolog protein DHP2, DNase I like 3, DNASE1L3, Liver and spleen DNase, LS DNase, LSD

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, Raji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11330-1-AP targets DNASE1L3 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNASE1L3 fusion protein Ag1873 相同性解析による交差性が予測される生物種
Full Name deoxyribonuclease I-like 3
Calculated molecular weight 305 aa, 36 kDa
Observed molecular weight 33-38 kDa
GenBank accession numberBC015831
Gene Symbol DNASE1L3
Gene ID (NCBI) 1776
RRIDAB_2095527
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13609
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Deoxyribonuclease 1-like 3 (DNASE1L3, DNase gamma, DNase Y, LS-DNase) is member of a DNASE1 protein family that is identified by similar biochemical properties such as Ca2+/Mg2+ dependency and an optimal pH of about 7.0 as well as by a high similarity in their nucleic acid and amino acid sequences [PMID:15796714]. It is an endonuclease and cleaves double-stranded DNA (and single-stranded DNA) producing 3'-OH/5'-P ends, which is Ca2+/Mg2+-dependent and inhibited by Zn2+, but not by monomeric actin [PMID:9665719]. In addition, it has been suggested to be involved in apoptotic DNA fragmentation of thymocytes neuronal PC12 cells, C2C12 myoblasts and of cell lines expressing the recombinant nuclease [PMID: 7957253, 9307016]

Protocols

Product Specific Protocols
WB protocol for DNASE1L3 antibody 11330-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols