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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DNASE1L3 Monoclonal antibody, PBS Only

DNASE1L3 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A4D4

Cat no : 67041-1-PBS

Synonyms

Deoxyribonuclease gamma, deoxyribonuclease I like 3, DHP2, DNAS1L3, DNase gamma, DNase I homolog protein DHP2, DNase I like 3, DNASE1L3, Liver and spleen DNase, LS DNase, LSD

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using DNASE1L3 Monoclonal antibody (67041-1-Ig)

    WB analysis using 67041-1-Ig (same clone as 67041-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67041-1-Ig (DNASE1L3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67041-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using DNASE1L3 Monoclonal antibody (67041-1-Ig)

    WB analysis using 67041-1-Ig (same clone as 67041-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67041-1-Ig (DNASE1L3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67041-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Daudi cells using DNASE1L3 Monoclonal antibody (67041-1-Ig)

    WB analysis of Daudi using 67041-1-Ig (same clone as 67041-1-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 67041-1-Ig (DNASE1L3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67041-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using DNASE1L3 Monoclonal antibody (67041-1-Ig)

    IHC staining of mouse liver using 67041-1-Ig (same clone as 67041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67041-1-Ig (DNASE1L3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67041-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using DNASE1L3 Monoclonal antibody (67041-1-Ig)

    IHC staining of mouse liver using 67041-1-Ig (same clone as 67041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67041-1-Ig (DNASE1L3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67041-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using DNASE1L3 Monoclonal antibody (67041-1-Ig)

    IHC staining of human spleen using 67041-1-Ig (same clone as 67041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67041-1-Ig (DNASE1L3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67041-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using DNASE1L3 Monoclonal antibody (67041-1-Ig)

    IHC staining of human spleen using 67041-1-Ig (same clone as 67041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67041-1-Ig (DNASE1L3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67041-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67041-1-PBS targets DNASE1L3 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen DNASE1L3 fusion protein Ag28187 相同性解析による交差性が予測される生物種
Full Name deoxyribonuclease I-like 3
Calculated molecular weight 305 aa, 36 kDa
Observed molecular weight 33-38 kDa
GenBank accession numberBC015831
Gene symbol DNASE1L3
Gene ID (NCBI) 1776
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

Deoxyribonuclease 1-like 3 (DNASE1L3, DNase gamma, DNase Y, LS-DNase) is member of a DNASE1 protein family that is identified by similar biochemical properties such as Ca2+/Mg2+- dependency and an optimal pH of about 7.0 as well as by a high similarity in their nucleic acid and amino acid sequences (PMID:157967140). It is an endonuclease and cleaves double-stranded DNA (and single-stranded DNA) producing 3′-OH/5′-P ends, which is Ca2+/Mg2+-dependent and inhibited by Zn2+, but not by monomeric actin (PMID:9665719). In addition, it translocates from the endoplasmic reticulum to the nucleus during apoptosis (PMID: 23229555). DNASE1L3 can be located either in the cytoplasm or in the nucleus.