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DNASE2 Polyclonal antibody

Name: DNASE2 Polyclonal antibody
Brand: Proteintech
SKU: 15934-1-AP

DNASE2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15934-1-AP

Synonyms

DNASE2A, DNase II alpha, Deoxyribonuclease-2-alpha, Deoxyribonuclease II alpha, Deoxyribonuclease 2 alpha

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of HeLa using 15934-1-AP
WB result of DNASE2 antibody (15934-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNASE2 transfected HeLa cells.
WB analysis using 15934-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15934-1-AP (DNASE2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis using 15934-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15934-1-AP (DNASE2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of U-87 MG using 15934-1-AP
IP result of anti-DNASE2 (IP:15934-1-AP, 4ug; Detection:15934-1-AP 1:500) with U-87 MG cells lysate 1160 ug.
IP experiment of HeLa using 15934-1-AP
IP result of anti-DNASE2 (IP:15934-1-AP, 4ug; Detection:15934-1-AP 1:500) with HeLa cells lysate 1360 ug.
IHC staining of mouse kidney using 15934-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15934-1-AP (DNASE2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 15934-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15934-1-AP (DNASE2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 15934-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DNASE2 antibody (15934-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HeLa using 15934-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DNASE2 antibody (15934-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Cat No. 15934-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, LNCaP cells, Jurkat cells, U-87 MG cells, MDA-MB-231 cells
Positive IP detected in	U-87 MG cells, HeLa cells
Positive IHC detected in	mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 5 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

15934-1-AP targets DNASE2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DNASE2 fusion protein Ag8781 相同性解析による交差性が予測される生物種
Full Name	deoxyribonuclease II, lysosomal
Calculated molecular weight	360 aa, 40 kDa
Observed molecular weight	32-40 kDa
GenBank accession number	BC010419
Gene symbol	DNASE2
Gene ID (NCBI)	1777
RRID	AB_2095546
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DNASE2, or 'acid DNase,' hydrolyzes DNA under acidic conditions and is one of 2 distinct mammalian DNases. It hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. It has a key role in the degradation of nuclear DNA in cellular apoptosis during development [PMID:18812394]. Besieds, it is necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells. It has been demonstrated to be responsible forDNA degradation in apoptotic cells engulfed by macrophages and exogenous DNA on the skin surface [PMID:23019102]. DNASE2 exists some isoforms with MV 39 and 34 kDa. (PMID: 14644493)

Protocols

Product Specific Protocols
WB protocol for DNASE2 antibody 15934-1-AP	Download protocol
IHC protocol for DNASE2 antibody 15934-1-AP	Download protocol
IF protocol for DNASE2 antibody 15934-1-AP	Download protocol
IP protocol for DNASE2 antibody 15934-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Int Immunopharmacol Angelica sinensis polysaccharides ameliorate 5-FU-induced stress anemia via promoting extramedullary erythroblastic island central macrophage-mediated erythroid differentiation Authors - Biyao Wang View Article
mouse	WB	J Biol Chem Autolysosomal degradation of cytosolic chromatin fragments antagonizes oxidative stress-induced senescence. Authors - Xiaojuan Han View Article
human	WB	Pharmacol Res Oroxylin A regulates cGAS DNA hypermethylation induced by methionine metabolism to promote HSC senescence Authors - Danli Zhao View Article
human,mouse	WB	Mol Nutr Food Res Mangiferin protects DNase 2 abundance via Nrf2 activation to prevent cytosolic mtDNA accumulation during liver injury Authors - Lisi Li View Article
mouse	WB,IHC,IF	Transl Neurodegener Neuronal double-stranded DNA accumulation induced by DNase II deficiency drives tau phosphorylation and neurodegeneration Authors - Ling-Jie Li View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DNASE2 Polyclonal antibody

DNASE2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of HeLa using 15934-1-AP

WB analysis using 15934-1-AP

WB analysis using 15934-1-AP

IP experiment of U-87 MG using 15934-1-AP

IP experiment of HeLa using 15934-1-AP

IHC staining of mouse kidney using 15934-1-AP

IHC staining of mouse kidney using 15934-1-AP

IF Staining of HeLa using 15934-1-AP

IF Staining of HeLa using 15934-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Angelica sinensis polysaccharides ameliorate 5-FU-induced stress anemia via promoting extramedullary erythroblastic island central macrophage-mediated erythroid differentiation

Autolysosomal degradation of cytosolic chromatin fragments antagonizes oxidative stress-induced senescence.

Oroxylin A regulates cGAS DNA hypermethylation induced by methionine metabolism to promote HSC senescence

Mangiferin protects DNase 2 abundance via Nrf2 activation to prevent cytosolic mtDNA accumulation during liver injury

Neuronal double-stranded DNA accumulation induced by DNase II deficiency drives tau phosphorylation and neurodegeneration