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DNM1 Monoclonal antibody

DNM1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse, Rabbit, Pig, Chicken

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B2A11

Cat no : 68009-1-Ig

Synonyms

DNM, DNM1, dynamin 1

Validation Data Gallery

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  • Western Blot (WB) analysis of pig brain tissue using DNM1 Monoclonal antibody (68009-1-Ig)

    WB analysis of pig brain using 68009-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 68009-1-Ig (DNM1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using DNM1 Monoclonal antibody (68009-1-Ig)

    WB analysis of A549 using 68009-1-Ig

    WB result of DNM1 antibody (68009-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNM1 transfected A549 cells.

  • Immunohistochemistry (IHC) staining of rat brain tissue using DNM1 Monoclonal antibody (68009-1-Ig)

    IHC staining of rat brain using 68009-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68009-1-Ig (DNM1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using DNM1 Monoclonal antibody (68009-1-Ig)

    IHC staining of rat brain using 68009-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68009-1-Ig (DNM1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Neuro-2a cells using DNM1 Monoclonal antibody (68009-1-Ig)

    IF Staining of Neuro-2a using 68009-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using DNM1 antibody (68009-1-Ig, Clone: 1B2A11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inpig brain tissue, A549 cells, Rabbit brain tissue, Rat brain tissue, Mouse brain tissue, Chicken brain tissue
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNeuro-2a cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68009-1-Ig targets DNM1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Rat, Mouse, Rabbit, Pig, Chicken samples.

Tested Reactivity Human, Rat, Mouse, Rabbit, Pig, Chicken
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DNM1 fusion protein Ag12932 相同性解析による交差性が予測される生物種
Full Name dynamin 1
Calculated molecular weight 851 aa, 96 kDa
Observed molecular weight100 kDa
GenBank accession numberBC063850
Gene symbol DNM1
Gene ID (NCBI) 1759
RRIDAB_2918756
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DNM1 antibody 68009-1-IgDownload protocol
IHC protocol for DNM1 antibody 68009-1-IgDownload protocol
IF protocol for DNM1 antibody 68009-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols