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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DNM2 Monoclonal antibody (68209-1-Ig)

    WB analysis using 68209-1-Ig (same clone as 68209-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68209-1-Ig (DNM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68209-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DNM2 Monoclonal antibody (68209-1-Ig)

    IF Staining of HeLa using 68209-1-Ig (same clone as 68209-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DNM2 antibody (68209-1-Ig, Clone: 1B8H3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68209-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50954-1

    Cytometric bead array standard curve of MP50954-1

    Cytometric bead array standard curve of MP50954-1, DNM2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68209-1-PBS. Detection antibody: 68209-2-PBS. Standard:Ag6274. Range: 0.781-100 ng/mL.

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DNM2 Monoclonal antibody, PBS Only (Capture)

DNM2 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 68209-1-PBS
Clone No.1B8H3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

dynamin2, Dynamin II, dynamin 2, DYN2, DNM 2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68209-1-PBS targets DNM2 as part of a matched antibody pair:

MP50954-1: 68209-1-PBS capture and 68209-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DNM2 fusion protein Ag6274 相同性解析による交差性が予測される生物種
Full Name dynamin 2
Calculated molecular weight 98 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC054501
Gene Symbol DNM2
Gene ID (NCBI) 1785
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP50570
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

DNM2, also known as DYN2, DYNII, CMTDI1, CMTDIB, belongs to the dynamin family. DNM2 is a ubiquitously expressed large GTPase involved in clathrin-dependent and -independent endocytosis and intracellular membrane trafficking. DNM2 plays a role in the regulation of neuron morphology, axon growth, and the formation of neuronal growth cones. Dynamins are associated with microtubules. DNM2 plays an important role in endocytosis and is involved in cytokinesis (PMID: 33713620, 12498685). Mutations in DNM2 cause dominant centronuclear myopathy (PMID: 16227997). Defects in DNM2 are the cause of Charcot-Marie-Tooth disease dominant intermediate type B (CMTDIB) (PMID: 15731758). Alternate splicing results in multiple transcript variants.